Pharmacognosy And Quality Specification Studies On Leaf Of Rehmannia Glutinosa Libosch.

The leaf of Rehmannia glutinosa Libosch. Is the scrophulariaceae plant. Glutinous rehmannia's traditional for medicinal is the root tuber.The leaf usually burns down as the fertilizer, as to develop the advantage land yellow leaf resources fully,Investigate leaves′resources and variety condition of Rehmannia glutinosa Libosch.;the Pharmaconosy﹑ the quality specification and catapol's content change situation research in normal stores.Conducts the histology research used the optical microscope to the leaf through epidermis movie-making,unarmed slice,paraffin wax slicing as well as powder movie-making;The paraffin wax slicing carries on the organization,the cytology research to the leaf; The Rehmannia glutinosa Libosch. in the national distribution is widespread,and the variety is numerous;The leaf powder movie-making,may observe the glandular hair,the non-glandular hair(2～6 cells)and the spiral vessel;the 5%KOH dissociation movie-making,in the leaf vein arranges the close spiral vessel obviously;through epidermis movie-making,observed epidermal cell,blowhole,the blowhole shaft type has the straight axle type,the inequality (subsidiary cell 3～4,one is smaller compares other two), the infinitive(subsidiary cell 3～6,epidermal cell's shape similar)three kinds,the top and bottom epidermis all has the blowhole,blowhole arrangement to be crowded in epidermis below,above the epidermis is sparse.The latitudinal section,under the main Leaf vein the epidermis is composed obviously by 2 layers cells,other is composed of 1 layer,the main Leaf vein and the big side veins by Bundle and the collenchyma,the xylem located at to the stem surface,the xylem drive pipe are dyed red by the safranine,the bast located at the back stem surface,underneath Bundle has the collenchyma distribution;The mesophyll including the Palisade tissue and the Spongy tissue,the Palisade tissue located at on epidermis underneath,is composed of 1 row arrangement close oval cylindrical cell,in the Palisade tissue covers entirely disperses the punctual chloroplast,under the epidermis there is no Palisade tissue,the Palisade tissue does not pass the leaf vein;The Spongy tissue located at the Palisade tissue with under epidermis,is composed of some kind of circular's parenchyma cell,the intercellular space is big,arrangement loose like sponginess,the cell content's chloroplast compares the Palisade tissue to be few;The leaf epidermis movie-making, passes with the chloral hydrate passes,carries on the determination to its arteries island number,the arteries island number is 19～20.6～24,the Palisade tissue cell and epidermal cell ratio is 4.8～6.1～7.5,epidermis below stomatal index 17.2～21.2～23.6,epidermis above is 4.2～6.8～9.6 ,epidermis below stomatal index is bigger than above.Used highly performance liquid chromatography to carry on the contents determination to its main effective component;Taked the catalpl as the target,Used orthogonal experiment to arrange and to carry on the experiment,definite optimum extraction craft on leaf;has studied under the room temperature storage conditions,catalpal′s change rule.the optimum extraction craft is:The water decoction extraction,2 times,each time 1h In the orthogonal;To six variety yellow leaves content determination result is:Beijing No.1 is the most high of 5.63%, Beijing No.3 is the most high of 2.28%,and content has the obvious difference in the different varieties;Through the room temperature storage condition content′s change research,discovered that 7,8,9 three monthes catalpol′s content drops obviously.The variety is numerous and the distribution is widespread,now only Henan producing is the best,the ecliptic nature research can a better promotion to its science planter and the management;The leaf has the unique micro characteristic,has the important distinction significance,its characteristic may take the character appraisal and the quality specification basis ;Through the nature storage conditions research,demonstrated that the content drops obviously,possibly receives the temperature humidity effect;Provides the essential theory basis for leaves of Rehmannia glutinosa Libosch. comprehensive development use.