Theoretical And Applied Studies On Microbial Transformation Of Fructus Arctii

Fructus arctii, the seed of Arctium lappa L., is one of the familiar Chinese traditional medicines. The chief effectual components of fructus arctii are arctiin and arctigenin. Actually, arctigenin is one of the most effectual components of fructus arctii, which has obvious effects, such as anti-cancer, anti-virus, calcium antagonist actions and platelet activating factor antagonist activity.Arctiin belongs to lignin, which is easily isomerized by acid or alkali. Therefore the chemic method of transforming arctiin is deficient. Microbial method was adopted to transform arctiin with the enzyme excreted by microorganism, which could solve the problems of the chemic method. The pure product was assayed by NMR,IR and MS.The main concerns and results of researches in this study have been summarised:I. A strain with high effective transformation of arctiin was screened, named as X3-1;II. Optimization for the ferment conditions include:1. Culture medium of the germ: lixivium of corn 25%, lixivium of Fructus arctii 6%, solution of salt 1%;2. Culture medium of the ferment: extract of fructus arctiiv (farina: water=1:10, w: v), unaffected pH.3. The temperature for the enzyme was 60℃and the pH was 6;III. Fermentations of fructus arctii were performed in a 5L fermentor (FPC2000 glass fermentation tin, Korea) containing 3.5L of the medium. The tests were carried out at 30℃, 200 rpm for agitation. The aeration rate was 1.5v.v.m.; and the culture time was 168h; IV. After being added 10% NaCl and adjusted pH to 5.5, resin D101-I was used to adsorb the fermentation liquor which was concentrated three times with a flow velocity of 3BV/h. Then it was washed at a velocity of 2BV/h using acetone and ethanol (85:15, v: v) and stopped when arctigenin could be analyzed in the outflow by TLC. The sorption and desorption rate were 56.1mg/g and 89.2% respectively.