Selection Of Pleurotus Ostreatus Asexual Regenerative Strains By Protoplast Regeneration

Pleurotus ostreatus is an important type of edible fungus,it is strong adaptability, widely cultivated sources of raw materials,high yield and efficiency with rapid development in recent years,it has become a popular vegetable fungi for the urban and rural people.However,due to long-term asexual breeding,the Pleurotus ostreatus industry development is faced the apparent degradation,lack of resources,output is low and unstable,a single breeding of practical issues.In order to enhance the effectiveness and speed of the breeding new strains,the experimental breed the new strain of Pleurotus ostreatus by renewable protoplast technology,protoplast preparation and regeneration condition,renewable strains of screening,such as a series of re-screening test,purport from the cellular level to explore a new way Mushroom breeding for the development and improvement of food Selection of new strains provide the basis.Protoplast preparation and regeneration condition,renewable strains of screening,such as a series of re-screening test, purport from the cellular level to explore a new way mushroom breeding for the development and improvement of food selection of new strains provide the basis.1.We have studied the condition of the preparation of Pleurotus ostreatus protoplast in many details and it shows that using the mycelia cultrued in PDA medium at the age of 5 days,2%lywallzyme or the mixture of 1%cellulas and 1%snailas under the condition as 30℃,3h enzyme action with 0.6mol/L MgSO₄ as osmotic stabitizor,pH is 6.5 is the suitable condition of preparing protoplast ofPleurotus ostreatus.The Pleurotus ostreatus the age of hyphe was 3 days,the digesting time wasl hour,the osmotic stabilizer was 0.6 mol/L MgSO₄.the frequency of regeneration was the best and resch up 4.35%.2.Under the best condition of separation and regeneration,we get 98 regenerative strains,.Observed under the microscope,47 strains have clampconnection.The result of antagonist response shows that 39 strains are different from original strains.3.Through mycelial color,mycelial growth,mycelial density compared experiment,from 39 regenerative strains we get some strains such as 7,11,13,17,26,36,37 have stronger growing trend,biger density than original strains and they grow more quickly.Different cloture spawn has different growing state,and they have positive correlation with mother cultures stains.4.Compared with contaminated rate between regenerative strains and original strains,1,3,7,14,18,24,25,26,30,33,37,38 have stronger ability of anti-bacteria capacity.It can be used as the basis for further selection.