Study On The Tissue Culture Of Sambucus Williamsii Hance

Sambucus williamsii Hance is a deciduous shrub of the capnfoliaceae, which widely distributed in northern China. It is a kind of valuable tree for it's more economic, social and ecological benefits. Presently, Sambucus williamsii Hance are mainly bred through sowing seeds and the cutting, but these ways have difficulty to satisfy the production and the application need. In order to speed up the pace of introduction and artificial cultivation, produce high-quality seedling, large scale production and promote new superior varieties, seedling stems with axillary bud, leaves and other organizations of Sambucus williamsii Hance were used as explants to conduct in vitro culture .The main results are as follows:1. The axillary buds were quickly induced from stems under the medium MS + NAA 0.2 mg. L^-1 + 6-BA 2.0 mg. L^-1 + sugar 30 g. L^-1+ agar 6 g. L^-1, and after 15 days, the robust buds can be gotten .The optimum multiplication culture medium for the buds was MS +TDZ 0.03 mg. L^-1 + 6-BA 2.0 mg. L^-1 + sugar 30 g. L^-1+ agar 6 g. L^-1, and after 25 days multiplication coefficient was 3.8. The highest induction frequency of roots could be gotten in medium 1/2MS + IBA 0.2 mg. L^-1 + 6-BA 0.1 mg. L^-1+ sugar 30 g. L^-1 + agar 6 g. L^-1, and the rate reached 100%. The root's growth was better and the length of district root hairs was 2.0 cm. When transplanted in pots with the mediems of 1 perlite : 2 humus, most of the little tree can grow well.2. It was easy to induce higher quality of callus from leaves and new tree top stem section of Sambucus williamsii Hance. The most appropriate medium was MS + 6-BA 0.1 mg. L^-1+ 2, 4-D 0.5 mg. L^-1 + sugar 30 g. L^-1+ agar 6 g. L^-1 + PVP 0.1 g. L^-1, on which well grown callus could be induced in great number in 30-40 days. To keep the hearty vigor and differentiation ability of callus, and 2, 4-D was gradually replaced by NAA, MS + 6-BA 0.5 mg. L^-1+ NAA 1.0 mg. L^-1+ sugar 30 g. L^-1+ agar 6 g. L^-1 + PVP 0.1 g. L^-1 was most suitable for multiplication of callus with higher multiplication coefficient and healthy growth. The adventitious buds had not been induced from callus. Further research should be conducted in the future.