| Studies were carried out to find out the best composition of medium and propagation procedure by using the explants of terminal buds, axillary's buds, leaves of Ardisia Mamillata Hance. The results were as follows:1)Culture conditions: temperature 25 2 , 14 hours illumination every day, and about 1500 Lux illumination intensity.2)The optimal explants were young leaves (with partial main vein), shoot tips of terminal buds or lateral buds in middle March.3)Effective surface sterilization of the primary explants was to soak them in 0.1% mercuric chloride for 8 minutes after washed in tap water for 1 ~2 hours and sterilized in 75% alcohol for 30 secends. Bloting the cultured material could prevent browning effectively when inoculating it on callus-inducing culture medium.4)Primary cultivation: The best medium for bud's sprouting in initiation culture was 2/3MS+BA2mg/L+IBA0.2 mg/L in which sprouting rate could reach 91%. The best Callus-inducing culture medium was MS+BAlmg/L+2,4-D1.0mg/L or MS+ BA0.2mg/L +NAA0.2 mg/L +2, 4-D 0.5mg/L. Callus-inducing rate were 75.19% and 67.72% respectively.5)The best subculture medium was MS+BA1.5mg/L+NAA0.1 mg/L+sucrose3%.Where the multiplication rate was 6.6 or 2.8 108 per year.6)The best rooting medium was l/2MS+IBA0.5mg/L+NAA0.5 mg/L +sugar2% +0.2%AC. Where the rooting rate could reach 93.6%.7)Training seedling: The survial rate could reach 64.4% when the hardened rooted test tube plants were transplanted into the medium with 1/2 vermiculite and 1/2 ruby mica.
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