Study On Tissure Culture And Plantlet Regeneration Of Anthurium Andraeanum

Anthurium andraeanum, a kind of perennial herb which belongs toAnthurium genus of araceae family, is generally used as cut flowermaretials because of its peculiar patterns and the long flowering period.Potted plants are also gradually favored by consumers and widely used asindoor decoration flowers due to their beautiful plant types. Since it’s toodifficult to meet the flower market demand for Anthurium andraeanumusing traditional breeding methods, plant tissue culture techniques isapplied to produce a large number of Anthurium andraeanum seedings.In this study, four excellent kinds of potted Anthurium andraeanumspeicies were chosen as experimental materials, and the influencing factorsin four culture stages that contain callus induction and differentiation,subculture of tube seeding, rooting and transplanting were systematicallyanalyzed. The main results of this study are listed as follows.1. The results of initial culture showed that leaves and petioles werethe two best explants among leaves, petioles, lateral buds, inflorescencesand spathe; The better effect of sterilization was5min with0.1%HgCl₂with the leaves and petioles as the explants. The best material period wasthe tender leaves unfolded during5-8d without the main vein,as well as thepetiole with unexpanded leaf in morphological top.1/2MS as basic medium,added the anti-browning agent PVP300mg/L can effectively reduce therate of leaf browning. There were no significant difference in the browningrate of leaves and petioles between the plants cultured in the dark and12 h/d lightness, but the colors of the callus induced were different, yellowcallus could be induced in the dark conditions and would transfer green inthe light training.2. The results of subculture of callus revealed that cytokinins andauxin concentration combination was the main factors that influence callusinduction and diversification. The interaction effect of the combination of2,4-D and cytokinins was better than the combination of NAA or IBA.Thecombination of2,4-D and cytokinins ZT or TDZ was obviously better than6-BA. Although callus could be induced in the culture medium withadditional6-BA2.0mg/L and2,4-D0.2mg/L in the initial generations, thebrowning phenomenon was serious during the subculture process, and theadventitious buds were not differentiated. The growth and differentiationstate of callus were better in the culture medium with ZT2.0mg/L or TDZ4.0mg/L.3. There were great differences of adventitious bud differentiation andregeneration of callus between different Anthurium andraeanum speiciesand explants（leaves and petioles）. The plant regeneration capacity ofAnthurium andraeanum speicies SAM and SST were stronger than SDM orSHG, and the leaves regeneration capacity of the speicies SAM, SDM,SHG was stronger than petioles, while the petioles regeneration capacity ofspeicies SST was on the contrary. The best medium of callus induction anddifferentiation for the blades and petioles of SAM and SST was1/2MS+TDZ4.0mg/L+2,4-D0.2mg/L, and for the blades of SDM andSHG, the appropriate medium was1/2MS+ZT2.0mg/L+2,4-D0.2mg/L,but only callus could be induced and no adventitious bud weredifferentiated with petioles as the explant.4. In the stage of tube seedlings subculture, the culture effect wasbetter using the callus with2-4buds as the explants, the propagation coefficient could reach14.4, and the plant height was2.98cm. The growthstate of tube seedlings was good in the culture medium of1/2MS+6-BA1.0mg/L+NAA0.3mg/L or IBA0.3mg/L. Tube seedlings subculture on thehormonal conditions decreased, appropriate to reduce the concentration ofsucrose did not affect the proliferation culture.5. Along with the increased number of subculture, the growth potentialof tube seedlings decreased, and using organic additives of CH100mg/L orYE50mg/L could promote the growth of tube seedlings. In the stage ofrooting culture, the appropriate culture medium for rooting was1/2MS+NAA0.1mg/L, and the regenerated plants grew well.