The Effect Of Different Forage To Concentrate Ratio On Rumen Fermentant And Microflora Of Inner Mongolia Cashmere Goat

6 the similar old Inner Mongolian white Cashmere wethers with permanent ruminal and proximal duodenum cannulas were divided into 2 groups of 3 and fed the diets of 2:8 concentration: forage ratio (groupâ… ) and 3: 7 concentration: forage ratio (groupâ…¡) respectively to study the effect of different concentration: Forage ratio of the diets on the rumen microflora, the rumen fermentation and the passage in digestive tract for investigating their associations. This study included 2 parts.Part one was to study the effects of diets of different concentration: forage ratios on the rumen fermentation and the fibre passage in digestive tract of the Inner Mongolian white Cashmere goats. The rumen fermentation indexes and the disappearance of the NDF in the two groups were determined.The result showed that the pH value, the acetic acid, the acetic acid to propionic acid ratio and the concentration of total VFA increased; however, the concentration of ammonia nitrogen, microbe protein and the propiopic acide decreased with the increasing of the forage in the diets, but the groups were not significantly difference ( P>0.05), the disappearance of the NDF in the rumen inceased with the increasing of the forage in the diets, but the the groups were not significantly difference (P>0.05).Part two was to study the effects of different concentration: forage ratios in the diets on the rumen microflora of the Inner Mongolian white Cashmere. First the total DNA of the rumen contents microbes were extracted through the improved bead crushing process, on which the UV spectrophotometer and the gel electrophoresis were performed and the results showed the DNA concentration was between 245 and 290ng/ul, the OD value was between 1.62 and 1.66 and most of the DNA chains were above 15,000 bp with complete structure. Secondly, Entodinium and 3 important fiber degradation bacteri in the rumen content of the two groups were quantified through SYBR Green fluorescent quantitation PCR with 16s/18SrDNA as the target sequence. The result showed that the Entodinium decreased; however, the Fibrobacter succinogenes, Ruminococcus flavefaciens and Ruminococcus albus increased with the increasing of the forage in the diets, but both of that was not significantly difference (P> 0.05).