The Gene Difference Of Quality Development During Seed Development Of Momordica Charantia. L And Extracte And Purify Of K-Polypeptide

This experiment carried on changes of morphological traits, physiologic indices and the accumulate trend of polypeptide-K during seed development of 33 M.charantia.L.Ascertain best time during seed development of seed.The results as follows:1,Cluster analysis based on morphological 28 traits was carried out on 33 M.charntia germplasm accessions.Results indicated that these 33 accessions could be grouped into three major clusters,namely wild type (clusterâ… ),small fruit type(clusterâ…¡)and large fruit type(clusterâ…¢);The three major clusters have conspicuous differences in morphological traits,all of them reflect morphological traits.All the morphological traits reflect conspicuous differences of genetype.The genetic distances among the accessions obtained from the same ecological zone are small,while wide genetic distances could be observed among the germplasm from different ecological zones.2,Twelve germplasm accessions of M.charantia were used in this experiment to study the relationship between the changes of fruit morphological traits and the stage of seed maturity.Germination percentage, seed dry weight and seed moisture content of different seed developing stage were determined.Results indicated that seed development of M.charatia could be classified into three stages:the rapid enlarging,the dry matter accumulation and the dehydration.Regularity changes developed during seed development of genotypic M.charantia.Seed development time of wild type M.charantia shorter,seed reached filling maturity after18DAA.Seed development time of long fruit type M.charantia longer than wild type M. charantia,it take 22DAA to reach filling maturity;Seed development time of small fruit type M.charantia longest in three major clusters,seed reached filling maturity after22DAA.The changes of fruit and seed morphological traits,Germination percentage,seed dry weight and seed moisture content of different during seed development,all of them display the differrence of genotypic.3,K-polypeptide was extracted from Momordica charantia seeds with vitriol and alkali fusion and initia appreciation with thin layerchromatography (TLC).The transport ratio is 0.288 extraordinary 0.29 called Khanna.B proteinum of Figure4-3 maybe K-polypeptide.4,The condicine of Bitter melon seed polypeptide sds-page had been improved,Results indicated that 12%saprate gel and 4%stack gel could saprate Bitter melon seed polypeptide best.