Plant Resistance Of Momordica Charantia L. To Aulacophora Cattigarensis Weise And Studies On The Plant Resistant Substances

Momordica charantia L.was harmed by the minority of phytophagous insects such as Bactrocera cucurbitae,Aphis gossypii Glover,Diaphania indica etc.during the whole growth period,which displayed strong resistance to lots of phytophagous insects.On insect-plant relationship and chemical defence theory,Momordica charantia L.contains resistant substances to pests which yielded by secondary metabolism.This paper validated the existence of resistant substances in leaves.The antifeeding effects of extracts on Aulacophora cattigarensis Weise adults were proved by bioassay.The main results are as follows:(1)Introducing the organic solvents extraction methods,the chloroform extract had the highest antifeeding activity among all the extracts extracted from the leaves of Momordica charantia L.against Aulacophora cattigarensis Weise adults.The antifeeding effect enhanced followed with concentration increased.The rate of antifeeding effects were 100%at 8mg/mL by selected and unselected test.The antifeedant effect treated after 48 hours was stronger than after 24 hours. (2)11 fractions were attained from chloroform extract by Silica Column Chromatography.K4 and K9 had the strongest antifeedant effect under unselected condition against Aulacophora cattigarensis Weise adults at 1mg/mL,and the antifeedant effect increased after 48 hours.(3)Volatile substances were collected by SPME(Solid-phase Microextraction).69 components were identified from it using GC-MS,the main components were 3-Hexen-1-ol,(Z)-(32.57%),Tetratriacontanel (6.34%),Palmitaldehydel(0.33%),Bicyclo[3.1.1]hept-2-ene-2-methanol, 6,6-dimethyl-(5.28%),gamma.-Tocopherol(3.86%).