The Research Of Molecular Marker-assisted Breeding About Volvariella Volvacea

In this study, 60 straw mushroom strains fruiting teste comparison, in accordance with agronomic traits selected 16 in the production and breeding of fine core of the relative strain, and the use of molecular markers of genetic distances were measured, and under different breeding goals, a group with four pairs combinations, have set up a straw mushroom germplasm evaluation and crossbreeding parent matching system.Through research the preservation of straw mushroom spore germination basidiospore relationship, we have the best single spore isolation spores storage temperature and time, that is, at 20°C spores stored one day. Single spore germination of spores from the best conditions, is 35°C incubator in the culture for 24 hours.Straw Mushroom is in the initial stage because of their common ancestry with edible mushrooms, with their single spore can be pregnant, straw mushroom production to solve the existing problems in the better improvement of straw mushroom varieties, straw mushroom single spore strains carried out a series of studies. Including single spore strains morphological characteristics and classification, the growth rate of mycelium are single spore strains fruiting capacity comparison. We found that single spore colony morphology of different strains, with diversity, according to colonies can be divided into main features of mycelia of Health developed dense gas and non-gas-Health hyphae crawl affixed to the growth medium of two. The fruiting test found that those who can fruiting single spore strains, the dense gas Health mycelium developed, fast growing and will be a large number of chlamydospore.Screening out of the single spore matching hybrid strains with pairs, at the junction of their colony mycelia for separation and sophisticated features colony observation, found no use of colony morphology to accurately identify hybridization of the importance of using molecular marker assisted breeding means to carry out. SCAR this study used molecular markers for the identification of heterozygous that SCAR marker than other molecular markers more reliable and stable.The germplasm resources through research, hybrid parent matching, single spore isolation, hybridization and hybridization pair of successful identification of a hybrid, although not good agronomy traits of the new varieties, but the establishment of this initial straw mushroom Same with fungi crossbreeding research system, I believe that as long as access to more single spore strains and hybrids of education they will be able to fine traits from the new varieties.