Study On Application Of Embryo Transplantation For Sheep In Winter

The technique of embryo transfer has been investigated in our country animal husbandry since the 19th century,and evoked nationwide people to study it. The perspective of the technique of embryo transfer are wide, meanwhile, the development is as good as expectation.This experiment was done in two areas of Heilongjiang Province since October in 2005 to December in 2007 for the development of the key technique of embryo transfer. The major research contents of sheep on estrus synchronization, superovulation, abdominal deep insemination and embryo transfer technology in the winter, such as the application of effects, and with the autumn sheep embryo transfer results compared.Experiment Method: Using the conventional method of embryo transfer, main research on the basis of body weight for different injection with different doses of FSH for superovulation in winter; with deep abdominal Insemination breeds for all of the donator in winter; on the different historical breeding conditions pregnant results were observed after being transplanted embryos receptor. And in winter sheep embryo transfer pregnancy rate, the level of egg fertilization rate and the average yield per available only in sheep embryo amount compared with autumn, study on the effect of embryo transfer in winter, and comparison results of embryo transfer in the autumn will be related, comparative data for analysis and comparison of autumn and winter embryo transplantation gap.The results of experiments indicated:1. In the 2007 winter embryo transfer test, for the recipients of superovulation ,all of the corepus luteum rates, eggs rates, useful embryo rates and transplant recipients pregnancy rates were significantly lower than in the fall of 2006 (P <0.05). But egg fertilization rate in 2007 winter was significantly higher than that in the fall of 2006, the difference was significant (P <0.05).2. In the 2007 Winter, with 180IU FSH superovulation treated for Do, Su and Tx, three embryos can be used in several varieties, egg fertilization rate, transplant recipients pregnancy rate among the area there was no significant difference (P> 0.05), in the area of egg recovery Su (93.33%) is the highest recovery rate in three varieties, and Tx (92.31%) close, but the difference was not statistically significant (P> 0.05), but significantly higher than that of Do (58.62%), the difference was significant (P <0.05).3. Transplant recipients pregnancy rate of 2007 winter (36.21%) was significantly lower than 2006's(54.50%), the difference was significant (P <0.05).