Studies On Cross-incompatibility Between Distantly Related Of Lily And The Methods To Overcome

Lily (Lilium) germplasm is rich in resources, culture has a long history, and the flower, the variety are gorgeous, extensive cultivation, can be used for cut flower decoration, garden green, and the lily bulb also has edible and medicinal value, making it a leader Flower Bulbs . Lily, Qingya refined and beautiful, aromatic and pleasant, regards as by the people chaste, bright, free and the happy symbol, is internationally one of very best-selling flowers and plants.Because our country lily breeding works start to be quite late, China nurtures has the proprietary intellectual property rights lily variety to be very few, global lily seedball market Holland occupies the major part the share, our country uses in the lily production the seedball major part coming from Holland at present, because north part of our country the majority regional climates are cold, this enables the overwhelming majority the lily variety which introduces from Holland to display its kind of nature. Therefore, breeds selectively under our country natural climatic environment condition can the normal growth lily new variety be a present our country lily breeding item has the very big practical significance.This research as tries the material take 10 lily varieties, use RAPD analysis technology the relationship of different varieties of the selected 10 lily cultivation, through the use of different methods of pollen viability to determine the different kinds of pollen viability, according lily varieties of color and the cold resistant match a hybrid combination and doing Hybrid test, the same type of genetic hybridization using conventional hybridization, between the Eastern lily and the Asian lily's distant hybridization, has used the different pollination method, has carried on the hybrid embryo's retrieval experiment to the remote hybrid embryo, obtained the following result: 1. Used the RAPD technical analysis to choose 10 lily different varieties to plant cultivates, the results showed that 10 materials can be divided into three categories, respectively for the Asian lily hybrid, the Oriental lily hybrid and the wild species, and the preliminary identification of all varieties relatives. Through clustering map and the similarity coefficients can be seen between 10 lily material's heredity similarity coefficients is between 0.483~0.756, O3 and A6 the similarity coefficients most to be small are only 0.483, explained that their heredity is away from far, the sibship is farthest, between A1 and A2 coefficient similar to 0.756, that their relationship recently.2. Pollen vitality test demonstration, The staining result overall is higher than the germination determination result, germination determination the highest is L.lancifolium, reached 88.41%, the lowest is Asia Lily Navona, only 22.01%, Asia Lily's average is 37.70%, Oriental Lily's average is 22.97%, from the average of germination determination the lily we can see the germination of Asia better than the Oriental lily. In the same type of genetic test results showed: between the Asia lily varieties'hybrid has a high ovary swelling rate and the average number seeds, but between the Oriental Lily varieties'hybrid has a few of ovary swelling, some do not expand, the hybrid seeds have not been obtained.3. Between the Asian lily and the Eastern lily hybridized has used the different hybrid method, Obtains through the comparison: the hybrid between Siberia and dazzling and the hybrid between Pollyanna and the Sorbonne are more suitable for cutting stigma pollination, the hybrid between Yelloween and Elite is more suitable for Mongolia leads the pollination, for the cutting stigma pollination between the Siberia and dazzling is the best performance, for the Mongolia leads the pollination between the Yelloween and Elite is the best performance, for the daubs 0.1%NAA for the stigma pollinating between the Siberia and dazzling and the hybrid between Pollyanna and the Sorbonne are the best performance.4. Determine the best hybrid embryo from embryonic period of 40 days after pollination.5. Taking O2×A3 as the example to the Asian lily and the Eastern lily hybrid embryo's medium has screened, in 70% ethyl alcohol antiseptic 60 seconds, and uses under again the 2%NaCl03 antiseptic 20 minute conditions, using ovule cutting raise, had determined the hybrid embryo sprouts the best medium formula is MS+NAA0.1mg/L+6-BA0.5mg/L+sucrose30mg/L.