Studies On The Technology Of Extraction And The Immunomodulation Effect Of The Achyranthes Bidentata Polysaccharides In Chicken

Polysaccharide is extensively distributed in cell membrane of animals,cell wall of plant and animalcule.Polysaccharide is natural polymer multipolymer that bonded by glucosidic linkage through aldose or ketose.It is the base of life.The plant polysaccharide is provided with important biologicalactivity.It entered into activities of cell in organism.It has the effects of treat acute leukemias,immunological enhancement,anti-inflammatory, antivirus,hypoglycemic action,reduce blood fat,antifatigue,fight against senium. Achyranthes bidentata Polysaccharides(ABP)is kinds of Polysaccharide with bioactivity distill from the dried root of Cyathula offinalis Kuan.The researsh of ABP was concertrated on determination of Polysaccharides content,separate and test of treat acute leukemias.Most investigation of ABP was concentrated in the determination of the separation and the experimental anti-tumor research.To make a deep study of ABP,the estraction technology and the immunomodulatory properties of ABP to be studied.The main results were as follows:1.The optimal condition of extraction is established by the method of orthogonal experiment:the temperatue of extraction is 100℃,the extraction time is 8h,the powder-to-water ratio is 1:20,and make esthanol concentration 95%.According to the above-mentioned conditions,the rate of ABP was 50.41%and the content were 69.33%.2.The effects of Achyranthes bidentata Polysaccharides(ABP)on sera antibody and blood biochemical values in chicken to be studied by animal test.After taken ABP, antibody leve of IgM,IgG and IgA in serum increased significantly(0.01＜p≤0.05), and the blood biochemical had no significant difference prepared with the control.ABP can elevate serum level to improve the immune ability of chicken,and all of the blood biochemical values were not affected significantly.3.The effects of Achyranthes bidentata Polysaccharides(ABP)on immunological of erythrocyte and lymphocyte in chicken to be studied.After taken ABP(400mg/kg),the ANAE positive rates of lymphocytes from peripheral blood in all the chicken were increased significantly compared with the control.The rates of rosettes test were increased significantly compared with the control in 21^stdays;The rates of T-lymphocyte rosettes test were increased significantly compared with the control,and presented the strength-effect relations.4.The effect of Achyranthes bidentata polysaccharides(ABP)on the immunologically competent cell and immune organ as fllow:The ANAE positive rates of T-lymphocyte and macrophage were increased significantly compared with the control group.This showed ABP could activate chicken immune organs and speedy growth of chicken immune organs.The number of T-lymphocyte and macrophage from immune organs were increased after taken ABP.ABP can affect the immunological function in chicken obviously.5.The effect of Achyranthes bidentata polysaccharides(ABP)on the the histology and ultrastructure of immune organs in the chicken to be studied.The immune organs(thymuses,bursa of Fabricius and spleens)were obtained.The paraffin section and hematoxylin and eosin(HE)staining of immune organ were used to observe the histomorphological change and the ultrastructural change of the structure of immune system.Compared with control group,the histological structure of fabricius was more perfect.Ruga of mucous was smooth and circumscription between cortex and medulla was earlier and clearer.The cortex of fabricius,thymic lobule thicken obviously,the diameter of lymphoid follicle was increased.The volume of splenic and the splenic nodule augmented,periaterial lymphatic sheaths thicken and the germinal center of splenic nodule were obvious;Circumscription between red pulp and white pulp was earlier and more obviously.The number of thymic corpuscle was increased.The lymphatic arrangement was closer and more mature than that in control group.The ultrastrucmral observation showed regular lymphatic in ABP,nuclear chromatin was increased and swinging,the number of nucleolus increased.The result showed that ABP could boost the early development of thymus,bursas of fabricius and splenic.