The Effect And Mechanism Of Achyranthes Bidentata Polysaccharides On Intestinal Tract In Immunological Stressed Piglets

Through setting up a stress model by one challenged with lipopolysaccharide (LPS) in piglets, the experiment studied the effects Achyranthes bidentata polysaccharides(ABPS) on intestinal structure and function with reference to related mechanism, anticipated elucidate whether ABPS couldalleviate growth inhibition and intestinal injury induced by LPS challenge in piglets. Forty four healthy cross bred Duroc X (Landrace×Largewhite) weanling piglets (24±1days-old,7.25±0.23kg BW) were randomly allocated to4treatments (normal control group and LPS control group, test group, each treatment10repeat,1piglet per replicate. Feeding the following diet, respectively, for24days. The feeding6days, formal period18days. The specific group plan is as follows: the first formal test1-15d, no LPS stimulation,The blank group, control group I and Ⅱ were fed the basal diet, the Ⅲ and IV group were fed basal diet supplemented with0.10%ABPS;After formal test16days, piglets in the control group II and group III were injected16respectively peritonealion80μ g/mL·BW lipopolysaccharide(LPS), whereas piglets in the control group were injected with normal saline the same dose. D-xylose was orally adminstrated to all piglets at the dose of lml/kg·BW after LPS challenge or saline injection. Blood samples were collected from precava vein at1h post-injection on16d. The samples of intestinal mucosa were collected after LPS injection3h and24h, snap frozen in liquidnitrogen, then stored at-80℃until analysis.The measurements and results were as follows:The influence of ABPS stimulation of lipopolysaccharide ileum piglets the Intestinal mucosa immunization barrierIn order to elucidate the mechanisms by which ABPS exerted its protective effects on intestinal structure and function, the experiment was conducted to investigate the effects of ABPS on the level of adenylate in intestinal mucosa, antioxidative ability and NO/NOS of ileal mucosa the concentration of D-xylose in blood and the diamineoxidase activity of Jejunal mucosa were measured after LPS injection3hours and24hours.The results indicated that dietary supplementation with0.1%ABPS improved SOD significantly (p<0.05), ABPS relieved the negative effects on growth performance caused by challenge of LPS,the MDA content and the bowel mucosa oxidation resistance of piglets were decreased by challenge of LPS. Therefore, bowel mucosa the oxidation resistance and NO/NOS system is affected by ABPS to play the protection of bowel mucosa.ABPS could increase the concentration of D-xylose in blood (p<0.05) and DAO activity in jejunal mucosa(p<0.05), decrease endotoxin content DAO activity in blood to some extent. These results indicate that ABPS can alleviate intestinal injury caused by LPS challenge.(2)The mechanism of protective effects of ABPS and function after LPS challenge on intestinal formThrough the measurement of DNA, RNA and protein in jejunal mucosa, intestinal Villus height, crypt depth, Villus height/crypt depth, lymphatic cell apoptosis, goblet cell number, research how ABPS improve the influence of intestinal mucosa ileum form by LPS.The results showed that the intestinal structure and function were damaged by LPS challenge,the ratio of RNA/DNA, intestinal Villus height and the number of goblet cell were decreased by LPS(p<0.05). ABPS could increase intestinal Villus height and the number of goblet cell, deepen crypt depth, increase the ratio of Villus height and crypt depth significantly (p<0.05).