Study On Pyramiding Multi-gene By Molecular Marker-assisted Selection To High Protein Content,Quality Subunit And Powdery Mildew Resistance Gene In Common Wheat

According to current wheat breeding goals in China,it is a mission to cultivate disease-resistant,high quality wheat new lines to wheat breeding.Therefore,nurturing a number of polymerization of wheat varieties,product quality,disease resistance and nutritional quality of wheat is a good one for the breeding of the moment.Identification of the phenotype of traditional methods is difficult to accurately select two more excellent genes individual.Application of molecular markers(molecular marker-assisted selection) can solve this problem,the rapid and accurate identification containing two more excellent genes polymers.This study was screened with high-protein gene GPC,and the quality subunit 5+10 resistance gene Pm21 molecular markers closely linked marker-assisted selection to further multi-gene polymerization breeding.The major results of the study are the following:1 High protein content(GPC) GPC-B1 gene controlling the protein content of wheat an effective gene.In this study,the material carrying the high-protein gene GPC-B1-A strain of wheat GPC,R-GPC,PF638741,PI638740(BC₆F₃) The father of four,and they do not have the gene in wheat varieties Guinong 775,Guinong 21,X-2003 F₂ your agricultural materials,such as 20 for females.They are configurated combinations.SSR markers with GPC-B1 protein gone analysis.From 12 pairs of primers were screened with a chain-B1 GPC Xuhw89₁₂₂ markings.On the X-2003/ PI638740 separated from the group as a test F₂ groups SSR analysis.The tested results show that the marker gene and protein GPC-B1 chain,and the specificity,good repeatability.They can be used effectively for GPC-B1 protein gone molecular marker-assisted selection.To further the protein content of wheat gene GPC expression of the protein content in the role of parents and F₁ hybrid for the determination of the protein content.The results showed that a high-protein gone of the protein content in the material PI638740 reached 20.41 percent.According to field experiment observation,the material of PI638740 mature about a week early more earlily than other wheat materials.The Guizhou local wheat protein content in general 12-19%,14-16%in the more protein content can reach over 18%of the material is very small.2 Subunits of wheat 1Dx5 and IDy10 is HMW component of the baking quality of wheat and related.Parents in the above material,and 5+10 subunit specific PCR markers known to carry a certification several 1Dx5 and 1Dy10 gene material.On the X-2003/PI638740 separatcd from the group as a test F₂ groups SSR analysis.The tested results show that the labeled with 1Dx5 and 1Dy10 chain gone.The results of the PCR is good reproducibility and specificity strong.Wheat for groups supporting improved quality traits choice of high efficiency,high-quality wheat for the molecular marker-assisted selection to provide a reliable basis.3 The powdery mildew resistance gone Pm21 is the best Pm resistance gone.With the use of the chain of SCAR marker gene known to contain Pm21 powdery mildew resistance materials,and X-2003/PI638740 separated from the group F₂ groups as a test of SCAR.The validation results that the marker chain with powdery mildew gene Pm21 and it is the specificity,good repeatability.They can be used effectively for powdery mildew resistance gene molecular marker-assisted selection.4 In this study,based on the polymerization of the hybrid used in the early generations were resistant identification and molecular marker-assisted selection strategy,a high-protein gene GPC-B1,quality subunit 1 Dx5,1Dy10 and powdery mildew resistance gene Pm21 in Screening.We screened the use of high-protein gene and GPC-B1 chain SSR and identification markings 5+10 subunit specific PCR markers, as well as closely linked with the Pm21 SCAR markings on the compound combinations X-2003/PI638740 the F₂ molecular marker-assisted selection. Screening 13 polymerization to a GPC-B1+(5+10)+Pm21 plant;16 polymerization with GPC-B1+(5+10) plant;42 polymerization with GPC-B1+Pm21 plants.These polymers wheat breeding future generations of Guizhou provide potential resources.5 The system of high-protein,high-quality disease-resistant wheat breeding of molecular-assisted selection was initially established,with the help of high-protein wheat,disease-resistant gene in wheat quality chain and the gene-related molecular markers.In the breeding process,It could be carried out the molecular marker-assisted selection on the early generations of high-protein content Gene GPC,quality and subunit 5+10 resistance gene Pm21,and it could greatly improve the accuracy of choice and future generations of hybrid and breeding efficiency,so as to shorten the breeding period,to speed up the breeding of wheat varieties speed...