| In order to afford the thereunder for rapid propagation of pear,we study on tissue culture and micrografting technology by using Pingguoli,S2,Xiangshuili and Shanli as the materials.Pear in vitro culture has some problems,such as low propagation coefficient and hard to regeneration from leaves.At the same time,we studied the effect of different age of explant on callus induction and differentiation.The results shows in the initiation culture,the suitable medium for Pingguoli was 1/3MS+6-BA1.0 mg·L-1+IBA0.2 mg·L-1+Vc75 mg·L-1+sucrose30 g·L-1+ agar6.5 mg·L-1,combination water culture for 4 days before inoculation.In the subculture stage, suitable medium for the proliferation of Pingguoli was 1/2MS+6-BA1.5 mg·L-1+ NAA0.3 mg·L-1+sucrose30 g·L-1+ agar6.5g·L1,the multiplication coefficient was 5.12.Suitable medium for the proliferation of S2 was MS+TDZ0.2 mg·L-1+GA2.5 mg·L-1+sucrose30 g·L-1+ agar6.5 g·L-1,the multiplication coefficient was 4.33.In the rooting stage,suitable medium for the rooting of Pingguoli was AS+ IBA1.0 mg·L-1+ sucrose 20 g·L-1+agar6.5g·L-1,and dark culture for six days,rooting rate was 63.33% .The transplant survival rate of Pingguoli plantlets and grafting plantlets were 55% which in the media of humus:river sand =2:1.The physiological age of explant had an important effect on callus induction and differentiation of Shanli.In the embryo culture for regeneration plant stage,the ability for callus inducement and differentiation of cotyledon which just end the cold treatment was significantly higher than the cotyledon which were cultured under light for 10 days.The first euphylla had more higher differentiation ability,but the follo wing leaves which from subculture have no differentiation ability.Study on micrografting technology,using rooting stem and rootless stem of Xiangshuli and Shanli as rootstock,stem tip of Pingguoli and Xiangshuili as scion. On the MS+BA1.0 mg·L-1+IBA0.3 mg·L-1+sucrose30 g·L-1+ agar6.5 g·L-1 medium ,the survival rate of Xiangshuli/Shanli(rootless) was 85%,Pingguoli/Shanli(rooting) was 95%.Grafting seedlings' rooting and the survival rate of Pingguoli/Xiangshuli (rootless) achieved as high as 86.7%and 54%on MS+BA1.0 mg·L-1+IBA0.3 mg·L-1+sucrose30 g·L-1+ agar6.5 g·L-1 medium.
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