Studies On Induction Of Callus And Its Differentiation Of Belamcanda Chinesis

Belamcanda Chinesis (L.)DC, whose roots and rhizomes are the most common Chinese herbal medicine, is a kind of perennial herbal plants from Irides. As natural resources can't fulfill the requirement of medicine and other industries, cultivation and tissue culture are considered to be a hopeful way to alleviate the crisis of natural resources. Tissue culture technique is a good method, which can control the growing condition of plants, accelerate the pace of propagation, and purify effectual element from cells. There are no reports about tissue culture on Belamcanda Chinesis.To establish a suitable plant regeneration system for genetic improvement of Belamcanda Chinesis (L.)DC by biotechnology, callus was induced from leaves,shoot-tips and caudex-like explant on MS media supplemented with auxins (2,4-D,NAA) or cytokinins(6-BA) at different concentrations. The explants included young leaves, shoot-tips, caudex-like and seeds.A sterilization procedure for the explants was developed in this study. Results indicated that the explants including young leaves, shoot-tips and caudex-like could be successfully sterilized by first 70% alcohol for 30 s and then by 2.0% NaClO for 15 min and 18 min. And the seeds was difficult to sterilize.Capability of the explants to form callus was dependent on the explant source and induction medium. The frequency of callus formation from the leaves and shoot-tips were 43.3% and 55.0% respectively. The optimal medium for callus induction of the leaves and shoot-tips was MS medium with 1.0 mg/L 6-BA and 2.0 mg/L 2,4-D in darkness.Adventitious shoot induction of the callus from shoot-tips was also carried on in this study. The optimal medium was MS medium with 2.0 mg/L 6-BA and 0.5 mg/L NAA under light.