Studies On Tissue Culture Of Paeonia Lactiflora Pall

Penoy have high ornamental and officinal values.This research was focused on the regeneration system establishment of peony by ways of clustered shoots and callus induction and solution of problems related to each stages of this process,providing scientific data for production on large-scale of peony.This varieties included:'Fenyunu','Zifengyu','Qiaoling','Taohuafeixue', 'Qingwen','Chunxiao'.This results are as below:(1)The best meterials for clustered shoots induction are tip buds and stem segments with axillary buds,which are appropriately sampled from February to April in spring.Soaking explants in 0.1%Hgcl₂ for 8min,etiolation treatment,low temperature and electronic vibration are effective for sterilization;Antibiotics added into media can inhibit contamination caused by endophytes.(2)The optimal for startup culture is 1/2MS+6-BA0.5mg/L +NAA0.1 mg/L +GA₃ 1.0mg/L;the optimal for proliferation is 1/2MS+6-BA1.0mg/L +IAA0.5 mg/L+ GA₃0.5mg/L.It is suggested that GA₃ be used during the induction of dormant buds.(3)During the proliferation process of peony 1/2MS+6-BA1.0mg/L+KT0.5mg/L can improve the growth status.TDZ and AgNO₃ inhibit induction of clustered shoots and growth of plantlets.(4)Callus induction from easy to hard is hypocotyls＞stem segment＞petioles＞leave.Only hypocotyl callus can regenerate plant;TDZ and 2,4-D was the best for callus induction,then is 6-BA and NAA,but 2,4-D restrain the plant regeneration.The better culture medium is 1/2MS+TDZ0.5mg/L+2,4-D0.2mg/Lor1/2MS+TDZ0.2mg/L+NAA0.5mg/L for leave,and for shoot induction callus is 1/2MS+TDZ0.1mg/L+2,4-D0.2mg/L;It was difficult for callus regeneration,which was worth further research.(5)The anti-browning can effectively control the tissue culture browning.Antioxidant must be used seriously as it may lead material distortion so as to affected growth and propagation.Accession 500mg/L PVP to the initial of explants and transition continuously 2-3 times can restrain browning. The control of browning is harder than at the early stage,but it can be overthroughed by using high thickness PVP(2000 mg/L) with several times of transition,and also non-effected growth and multiplication.