| Wanxi White Goose is a superordinary middle-typed goose.It mainly located in mountainous areas in the west of Anhui Province and Gushi County in Henan Province.It was identified as the country second class protected poultry and "national-level animal species resources Protection variety".In this study,growth characteristics of Wanxi geese were estimated by compared with production traits of Landaise Geese,which were received as an excellent variety all over the world,were expressed under crude fed.The test animal came from Wanxi white geese breed-conservation base in AnHui province,including 170 Wanxi white geese and 170 Langdaise geese.All the geese were fed together,ate and drank freely.Making reference to the best feeding standard,the diet for the test animal was designed as follows:metabolizable energy was 11.58MJ/kg and the crude protein was 18.16%during 1-28d;matabolizable energy was 10.99MJ/kg and the crude protein was 13.68%during 29-70d.The forage ratio was 3:1 from 29 to 56d,1:1 from 57 to 90d.20 Wan-xi white geese and 20 Langdaise geese each were chosen randomly to be sacrificed(half males and half females) after 12 hours fasting.Blood samples were collected via wing vein.The liver and pectoral muscle were put into 2ml centrifuge tube and preserved in fluid nitrogen.And the following slauger index should be detected including dressed weight,half-eviscerated weight,eviscerated weight,eviscerated weight index,liver weight,liver index,pectoral muscle weight and pectoral muscle index. Intramuscular fat was tested by the national standard method.The semi-automatic biochemical analyzer was used to test serum triglyceride(TG),total cholesterol(TCHO), high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol (LDL-C).The smart-SN-695B RIAγ,measuring instrument was used to test insulin(INS), free Triiodothyronine(T3),free Thyroxine(T4) level in serum.The total RNA was extracted from liver and muscle to measure the abundance of the growth hormone receptor (GHR),insulin like growth factor-1(IGF-1) and insulin like growth factor-1 receptor (IGF-1R) by Semi-quantitative RT-PCR usingβ-actin as an internal standard.The results are as follows:(1)In rough feeding conditions,the 90-day-old Wanxi White Geese liver and liver weight-rate were significantly higher than that of Langdaise Geese(p<0.01);But Dressed weight,Half-eviscerated weight,Eviscerated weight,Eviscerated weight Index,Pectoral Muscle weight,Pectoral Muscle index there was no significant difference between two breeds.(2) Intramuscular fat content in pectoral muscle of Wanxi White Geese was significantly higher than that of Langdaise Geese(p<0.01);Intramuscular fat content was no significant sex difference in two varieties(p>0.05).(3) Plasma TG,TCHO,HDL-C were exactly higher in Wanxi White Geese than those in Landaise Geese(p<0.01);in Langdaise Geese serum,levels of LDL-C and HDL-C there was a significant sex difference(p<0.01).(4) Plasma free T3 and INS were no significant difference between two varieties.The level of free T4 in Wanxi White Geese was exactly higher than that in Landaise Geese(p<0.01)(5) IGF-1 mRNA expression was higher in Wanxi White Geese(1.00±0.04) than that in Landaise Geese(0.78±0.04)(p<0.01).GHR and IGF-1R mRNA expression were no significantly different between Wanxi White Geese(0.93±0.05,0.81±0.07) and Landaise Geese(0.91±0.05,0.83±0.07)(p>0.05).(6).In Pectoral muscle,GHR,IGF-1 and IGF-1R mRNA expression were no significant difference between Wanxi White Geese(0.82±0.05,0.79±0.04,0.82±0.07) and Landaise Geese(0.84±0.05,0.77±0.04,0.82±0.07)(p>0.05).(7) Significant positive correlation was found among plasmaTG,TCHO,HDL-C in both of tow breeds.And significant positive correlation was also found between liver index and plasma TG in Landaise Geese.(8) Significant positive correlation was found between body weight and liver weight (r=0.35,p<0.05).The liver index with the expression of IGF-1mRNA in liver also had significant positive correlation(r=0.67,p< 0.O1 ).Significant positive correlation was found between body weight and Pectoral muscle weight(r=0.79,p<0.01 ).The Pectoral muscle weight with the expression of IGF-1 mRNA in Pectoral muscle also had significant positive correlation(r=0.57,p<0.05)Conclusion:under rough feeding conditions,there was a significant difference between two breeds only in the liver weight and liver index(p<0.01);In two breeds,it is resemble in metabolic mechanism of cholesterol but significant difference in the growth of liver and metabolic mechanism of TG;autocrine of IGF-1 maybe play an important role during the growth of liver and muscle in Geese.
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