The Epidemiology Of PRV GI And GB Antibody In Eastern China

Pseudorabies virus(PRV),or suid herpes virusâ… ,is a member of Alphaherpesvirinae, herpesviridae,which can cause Aujeszky's disease in swine,bovine,sheep and wild animals.PRV infection could cause its natural and main storing host-swine(also its main infectious origination) a huge loses,the most huge economical lose in all swine infectious diseases except Classic Swine Fever and Food and Mouth Disease.About 443 sera samples from east china during the four years from 2007 to 2008 were detected with the IDEXX indirect ELISA Kit which can distinguish the vaccination from wild-type virus infection. The serological data were analysed systematically.The results showed that the PRV infection is very popular in east china region.16.03%of positive rate was detected in nursery.About 34.65%of positive was detected in serum of boar,about 19.7%of positive was detected in serum of swine to raise a child,and about 16.59%of positive was detected in serum of adult swine.We had detected the immune antibody of PRV by using IDEXX PRV gB ELISA Kit,613 serum samples collected from 2007 to 2008 were detected.The results was assayed and induced,results showed comparison contrast that of different regions and different ages.Apparent difference was manifested in our results.And then,185 tissue samples which doubt to be PRV were detected by PCR.These samples were all collected from 2007 to 2008 in Eastern China.Results showed that 28.10%(52/185) of samples were positive in PCR detection,51.06%(24/47) of nurseries were positive in PCR detection.The results manifested that the PCR detection showed in our study is a good method to use to quick detection and epidemiological study.