Northern Parts Of The Pig Farm Wild Swine Pseudorabies Virus Infection Epidemiology

Pseudorabies (Pseudorabies, PR) is a pseudorabies virus (Pseudorabies virus, PRV) causes an acutehighly contagious disease. In clinical pigs mainly elevated, itching, neurological symptoms appear estrussows as temperature, repeated with infertility, lack of love and mummified, miscarriage, stillbirth, and aseries of reproductive disorders. In recent years, pseudorabies in recent years, the emergence of newdisease type: sows Kin rate is not high; piglets pigs intractable diarrhea and respiratory problems;fattening pigs with respiratory symptoms, is one of the reasons PRDC; disease infection to our bring hugeeconomic losses pig industry. In northern areas, the disease is often reported, but an upward trend. Inorder to understand the northern regions wild virus infection in pigs and pseudo-rabies epidemic situation,this study seven northern region for the study of large-scale farms, from the field epidemiology, serologicalsurveys (PR antibody levels and immune PR wild virus antibody level) and three aspects of molecμlarepidemiology to identify the prevalence of pseudorabies northern region, providing a scientific and rationalbasis for the region pseudorabies control, for which the research was conducted in the following aspects:Ⅰ. pseudorabies field epidemiological investigationThrough the northern parts of the pig pseudorabies often issued piglets, weaned piglets, fattening pigsthree stages to analyze a typical case, to pig farmers to provide reference for the correct diagnosis andtreatment of disease.II. Northern region7scale farms pseudorabies detection and analysis of immune antibody levelsThe application of pseudorabies indirect ELISA test kit for525porcine serum from the northernregion seven large-scale pig farms be immune antibody levels pseudorabies test resμlts positive rate was95.43%(501/525), where A, B, C, D, E, F, G farms were positive rate of97.3%,95.45%,95.92%,91.43%,94.2%,100%,94.5%, in addition to a relatively low D fields, the overall relatively high, ideallyantibody levels, through the different stages of the herd antibody positive rate shows the resμlts ofstatistical boars, sows, gilts reserve milk, piglets, weaned piglets, fattening pigs were positive rates were97.87%,98.98%,95.27%,94.29%,92.71%,93.94%.III. Northern regions7scale wild pig pseudorabies virus infection serological surveysThe test application pseudorabies gE-ELISA kit for525porcine serum from the northern region sevenlarge-scale pig farms carried pseudorabies field virus antibody levels, and the resμlts of the total positiverate was27.8%, A, B, C, D, E, F, G field of wild virus infection rates were20.27%,30.30%,58.16%,0,47.83%,29.69%,2.38%; sows, gilts, piglets (15-18days), public pigs, weaned piglets and fatteningpigs, the positive rates were35.71%,34.46%,22.86%,21.28%,21.21%and20.83%; the region boarsand sows PRV wild virus infection is more serious.IV. Northern Region three large-scale pig farms pseudorabies field virus purification testThis study selected the A, B, E3pig farms developed its purification scheme, the resμlts of theprogram to promote the use of the three farms, after a year of implementation of the program of purification, A field from front to purify purifying20.27%after8.7%, after5.88%B field from front topurify purifying30.30%,12.50%after the E field from47.83%before purification to purify, to performthis purification scheme, the purification achieved good resμlts, the survey the resμlts have importantpractical implications for other large-scale pig farms in northern guidance pseudorabies control andpurification.V. Northern parts of the pig farms infected with wild virus pseudorabievirus molecμlar epidemiologyThe application of PCR to investigate the scale of the northern part of the region from the acquisitionof the92farms suspected diseased testing, the resμlts of a total of37patients tested positive for thematerial, the positive rate of40.2%diseased. Sequence analysis showed that the current through the wholenorthern region has not yet appeared new pseudorabies field virus mutants. The measured sequenceisolates kinship with the Hebei region closer, which coμld have a direct relationship with Xinjiang inrecent years, from Hebei, Beijing and other places frequently introduction.