Effect Of Nano-Selenium On Dietary Aflatoxin B₁ Detoxification In Meat Ducks

200 one-day-old Sichuan ducks were randomly divided into five groups,and fed with conventional diets in the control group,80 ug/Kg AFB₁ in the other four groups,and nano selenium of 0.4 mg/Kg in groupâ… ,nano selenium of 0.8 mg/Kg in groupâ…¡,nano selenium of 1.6 mg/Kg in groupâ…¢.By clinic and pathological observation,detection of blood,hepatic function index,and the residual of AFB₁,and the studying of expression of CYP450 gene mRNA in ducks liver of different groups using the method of RT-PCR at the end of test,To study the effect of Nano-Selenium on dietary AFB₁ detoxification in meat ducks for 42 days.The results showed that,ducks of toxic group with 80 ug/Kg AFB₁ presented typical clinical symptoms.Chronic poisoning leaded to death and the ducks' mortality reached to 30%.The AFB₁ seriously affected growth performance and hematopoietic function,maded great damage on the liver and kidney function.Compared with the control group,the RBC,WBC quantity and content of Hb in blood decreased significantly.The contents of AKP,GPT,GOT,GR,GST,ADA enzyme in serum increased significantly,the contents of GR,GST,ADA in liver decreased significantly,Cells particles degenerated of duck liver by hepatic pathological observation.The residual of AFB₁ in serum,liver and muscle increased significantly.The activity of CY450 enzyme decreased significantly,the expression of CYP450 gene mRNA decreased significantly.Compared with toxic group,the toxic symptom of three nano-Selenium group obviously relieved, and showed a certain of detoxification.The RBC,WBC quantity and content of Hb in nano-Selenium group significantly higher than toxic group,The contents of AKP,GPT,GOT,GR, GST,ADA enzyme in serum higher than control group and lower than control group,The contents of GR,GST,ADA in liver lower than control group and higher than control group.Cells infiltrated of duck liver by hepatic pathological observation.The residual of AFB₁ in serum,liver and muscle decreased significantly.The activity of CY450 enzyme increased significantly,the expression of CYP450 gene mRNA increased significantly.Corn meal did fermentation test by inoculation of AFB₁ strain in 80 ug/Kg,and builded the model of chronic poisoning.The contents of GST enzyme in serum and liver increased with increasing test days,and were specific index of detoxifying effect in AFB₁ poisoning.In the experimentation,by the using of nanoselenium,the damage index of duck organism is well alleviated and organism's ability of antitoxic effect on AFB₁ is enhanced.The exact reason may be because that the using of nanoselenium could induce the organism's immunity,accelerate the antioxidation effect,and clear the accumulation of free radicals,protect the liver,etc.