The Research And Related Study Of Sepidemiology Of Bovine Mastitis In Shijiazhuang

In order to clarify the risk Probability of pathogen infection of bovine mastiffs in Shijiazhuang area,after the application of internationally accepted CMT being used for the entire group of test cows,the health cows,Subclinical Mastitis infecting cows and clinical mastiffs infecting cows had being determined.Taking milk in pool further,the traditional bacterial separatio,cultivation and identification methods have being used on milk samples for bacteriological identification.At the results of milk samples collected from the Shijiazhuang area(Xinhua District,Luquan,Lingshou,Xinle,Xingang,Pingshan,Shenze, etc.) were 206.After separation and purification on these samples,172 baeterias have being identified,Staphylococcus aureus 27.91%(48/172),Streptococcus 11.63%(20/172),E.coli 58.14%(100/172) are major pathogens in them.In the detected cows,21%of the cows were infected by one of bacterias,79%of cows were Infected by two or more of bacterias.The Staphylococcus aureus and E.coli are the main pathogens of Subclinical Mastitis Cows.8 E.coli,10 Streptococcus and 16 S.aureus have being detected for sensitivity test and hemolytic test.The results show that the three main types of bacteria have a variety of antibiotics resistance,just were more sensitive to Cyproterone two stars,norfloxacin, gentamicin,kanamycin small ammonia then others;The hemolytic ability of Staphylococcus aureus was stronger then the Two other bacterial which had weak or non-hemolytic on staphylococcal mastitis in sick cows during the illness,and had a strong virulence.92 milk samples had being gathered fiom Subclinical Mastitis cows,which were detected by using the gene amplification method and traditional method,respectively.58 samples of Staphylococcus aureus,16 samples of Streptococcus agalactiae and 46 samples of E.coli had being Identified by Conventional bacteria identification method.Samples of mixed infection by Salmonella,Streptococcus agalactiae,pathogenic yeast had being identified by multiple PCR detection.In this study,at the level of gene,the detection of pathogenic milk by multiple PCR is simple,rapid,high sensitivity,specificity and so on. The Minimal inhibitory concentration of three major pathogens bovine mastitis-Staphylococcus aureus,Streptococcus and Escherichia coli Sensitivity tests had being determined in vitro drug.The results show that the ultra-free powder of Chinese medicine group has good bacteriostasis to E.coli and its minimal inhibitory concentration(MIC) is 125mg/ml.The MIC to Staphylococcus aureus is 250mg/ml,while the MIC to Streptococcus agalactiae is 1000 mg/ ml or more.Rare Earth and amino acids,midazolam,phenanthroline,Schiff base,such as formation of the complexes have a good inhibitory effect.Purpose of the study is the synthesis of Eu(α-NMA) ³phen H₂O complex,and to explore the Eu naphthoic acid antibacterial properties and the role of the E.coli protein.Using agar diffusion method and the filter paper method (diameter 0.5cm) to determine the inhibition of the complex to E.coli,Staphylococcus aureus, Bacillus subtilis,yeast.Using protein electrophoresis(SDS-PAGE) methods to observe the complex protein in bacteria.The impact of ligand phen,rare earth ion Eu,the complexs between E.coli bacteria and membrane protein had being compared.We can see that the Eu and ligand complexes formed with the new features.Conclusion Eu naphthoic acid with broad-spectrum antimicrobial,and E.coli proteins have a greater impact.Technology should be taken to control the following HACCP by the experiment:â‘ The infection of pathogen in rearing environment should be controlled;â‘¡Contamination of the nipple should be prevented after milking;â‘¢The scientific and reasonable use of milking instruments reduce the probability of the incidence of mastitis;â‘£The spread of the disease caused by infected cow should be controlled;⑤Breast infection prevention and control during dry milk period;â‘¥Enhance the ability of anti-infective of organism by immunization;⑦Reduce the invasion of pathogenic microorganisms to the breasts by designing reasonable bed of cows;⑧Reasonable mandatory supervision mechanism must being set up and routinely check cow group,time to time monitor etc.⑨The use ofultrafine powder of Chinese medicine to combat mastitis cows should be recommend.