Biological Characters Of Wood Rot Fungi And Decay Capacity To Betulla Platiphilla And Gene Differential Display

It is a complex process in the wood which decayed by wood rot fungi. The gene expression of wood decaying is the complex and rigorous process, each genic function is different, control strictly and adjust the variety of physical and chemical properties. This paper study the mechanism of wood decay fungi on wood corrosion and chemical variety caused by wood decay. The study laid a theoretical foundation for the further study in different decadent period of wood rot fungi during the physiological state and gene expression. At the same time, the paper provide a theoretical basis on choosing decaying resist timbering and biological pulp. In this paper, the growing rate of five wood rot fungi in solid culture medium and their biomass in liquid culture medium was compared. The activity of three main ligninolytic enzymes in those fungi, such as Lip, MnP and Lac, were also tested. At the same time, these fungi were used to decay the wood samples from 300 natural trees of white birch, which to study their wood-decaying capacity by measuring wood weight loss. FTIR spectrometer analysis was used to measure infrared spectrograms of primitive birch wood sample and wood samples degraded by 4 species of fungi, research the effects of functional group. Meanwhile using mRNA differential display to screen the related gene of wood decayed by fungi, analyze the bioinformatics,gene function function, several aspect research were conducted as follows:1. Biomass and Biodegradability of 5 kinds wood decay fungiThe growing rate, biomass, ligninolytic enzymes characteristics and decomposing power of the fungi were incompletely correlation. The growing rate of Coriolus versicolor and Irpex lacteus were faster than that of Piptoporus betulinus and Forties fomentarius, Pholiota adiposa was the slowest. The biomass of Piptoporus betulinus was the highest, Coriolus versicolor, Irpex lacteus and Fomes fomentarius were placed in the middle, Pholiota adiposa is the lowest. The decomposing power of Coriolus versicolor to wood samples was better, and then were Fomes fomentarius and Piptoporus betulinus, Irpex lacteus and Pholiota adiposa were poor.2. Activity determination of 5 kinds wood decay fungiThere existed Lip, MnP and Lac activities in all fungi except Piptoporus betulinus, in which the activities of the enzymes induced by wood powder were all higher than that of the control. The Lac of Irpex lacteus and the Lip of Fomes fomentarius and Pholiota adiposa only expressed in wood powder medium, the longer the fungi were cultured, the higher the enzymatic activites was.3. Impact of functional groups on birch wood decayed by 4 kinds of wood decay fungiUsing 4 kinds of wood rot fungi (Piptoporus betulinus, Fomes fomentarius, Irpex lacteu, Coriolus versicolor ) decay white birch, analyse birch wood with FTIR. Results showed the function groups of wood and lignin were all degraded in different degree by wood white-rot fungi (Fomes fomentarius, Irpex lacteu, Coriolus versicolor). Although aromatic rings were not changed very much, side chains of lignin such as carbonyl groups, CH₂, syringic units and guaiacyl units were partly degraded. But wood decay by wood brown-fungi (Piptoporus betulinus) lignin was not degraded. The extents of lignin degraded by 3 species of wood rot fungi were different in the view of peak absorption intensity change, Fomes fomentarius and Coriolus versicolor had higher ability than Irpex lacteu in lignin degrading. Connect with the change of peak absorption intensity, cellulose and hemicellulose were all degraded.4. Studies on the gene expression of decay white birch or notChoose the Maoer Mt. Coriolus versicolor for subject and decay white birch wood of Maoer Mt. There were obtained 19 special expression fragments. There were 11 fragments before decay, 8 fragements after decay.