Differentially Expressed Proteomics Analysis Between Mature And Germinated Spores From Osmunda Cinnamomea L. Var. Asiatica

Osmunda cinnamomea L.var.asiatica is a kind of edible and medicinal herbaceous perennid in the genus Osmunda in Osmundaceae,whose spore germination is an important biology event for the sexual reproduction growth and life history cycle.At present,the molecule mechanism of spore germination is not well-known.We described the cytological features of mature and germinated O.cinnamomea spores, and compared the changes of proteome between them in this research.We obtained 1255 and 1375 protein spots on the 2DE gel from mature and germinated spores,respectively.Most of the protein spots in the two samples were distributed in pH5-6.5,and the majority of them were distributed in 30-80kD.By using the image analyse software,we obtained 101 differentially-expressed protein spots(represented 85 unique proteins) between mature and germinated spores.Of the 101 differentially-expressed protein spots,37 were up-regulated and 64 were down-regulated.These proteins can be assigned into 9 function categories respectively:(1) photosynthesis,(2)carbohydrate and energy metabolism,(3)transcriptional regulation-related, (4)membrane and transport,(5)protein metabolism,(6)amino acid metabolism,(7)cell structure, (8)signal transduction,(9)unknown proteins.Of them,the RuBisCo big subunit, phosphoribulokinase,cytochrome f,sedoheptulose 1,7-bisphosphate,S-adenosyl-L-homocysteine hydrolase,HSP90 and lutamine synthetase take part in photosynthesis, transcription regulation,protein folding and stress response respectively.These differently expressed protemics characteristics between mature and germinated spores provided protemics evidence for the further research of spore germination molecule mechanism,and also provided theoretical basis for the rapid reproduction of O.cinnamomea.