| In vitro and in vivo experiments were conducted to test the efficacy of three representative adsorbents (product A, main component is yeast cell extracts; product B, main component is HSCAS; product C, the compound of yeast product and HSCAS) to sorb aflatoxin B1 (AFB1). In addition, the character of adsorption was evaluated, utilizing adsorption isotherm and thermodynamic functions.In experiment one, in vitro sorption of AFB1 onto three adsorbents was characterized.⑴AFB1 binding capacity by three adsorbents at pH2.0, 6.0, 8.0, simulated gastric fluid and simulated intestinal fluid condition was compared.⑵AFB1 binding speed by three absorbents in vitro condition was compared.⑶The stability of absorbent-AFB1 complexes in vitro condition was conducted. Primary results indicated that:⑴Product B showed the highest in vitro affinity for AFB1, followed by product C and product A.⑵Product B bound 97.69 % of the AFB1 in solution in 10 min, and it remained over 96.03 % in 60 min at pH8.0. Product A and product C did not show so efficacy as product B.⑶Product B-AFB1 complex was much stronger than the other two kinds of complex in vitro condition. Therefore, three adsorbents all showed binding capacity for AFB1 in some extent in vitro condition and product B could bind AFB1 more effectively than product A and C.In experiment two, in vivo efficacy of three adsorbents as aflatoxin binders in male broilers exposed to aflatoxin-contaminated feed from 1 to 21 d of age was assessed. A total of 240 broilers were randomly allotted to eight treatments (three pens per treatment of ten broilers in each pen). As a result, feed intake (FI) and average daily gain (ADG) decreased (P<0.05) and feed gain ratio (F: G) increased (P<0.05) in the treatment fed aflatoxin-contaminated feed (AFB1: 98.8μg/kg) versus the control treatment. Serum total protein (TP), albumin (ALB), globulin (GLOB) levels were significantly decreased (P<0.05), activity of succinate dehydrogenase (SDH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in liver were repressed and malondialdehyde (MDA) content was increased (P<0.05) by AFB1. Product B (0.15 %) increased growth performance, increased serum protein levels and activity of SDH, SOD, GSH-Px, decreased MDA content (P<0.05). However, product A and product C did not show such effects. The data suggested that the three products tested alleviated the adverse effects on broilers caused by aflatoxin, but compared with product A and C, product B could alleviate the toxic effect of AFB1 more effectively.In experiment three, the character of product B actited on AFB1 was evaluated, utilizing adsorption isotherm and thermodynamic functions in vitro. Results showed that the adsorption process of product B fitted to the Langmuir isotherm, and there was no significant difference at different temperature. The data supported that the adsorption of product B on AFB1 is chemical interaction.In conclusion, product B, but not product A and C, could bind AFB1 effectively in vitro and in vivo. The adsorption is chemical interaction. These results indicated that product B could alleviate some of the toxic effect in broilers. It might prove be beneficicial in the management of aflatoxin-contaminated feedstuffs for poultry.
|
PDF Full Text Request