Study On The Bioactivity And Chemical Components Of The Extracts From The Mycelia Of Russula Cyanoxantha

As well as having many varieties,higher fungi are of high productive ability in secondary metabolites,containing prolific bioactivie substances such as terpene,flavonoid, phenolic,lactin and steroid,and so on.These substances with various and novel chemical structure are vital to develop durgs,health products and pesticides.For the past few years,the exploit of bioactivie metabolites from higher fungi were payed more and more attention at home and abroad.On the one hand,a lot of bioactivity metabolites from fungi with diverse structure were found continuously;on the other hand,the materials of mycelia could easily be obtained by fermentation.In this study,the shaking culture conditions of Russula cyanoxantha were optimized,the antioxidative and antitumer activities of the extracts from culture media and mycelia was tested,and then the component of ethanol extracts from mycelia of Russula cyanoxantha were purified,isolated and identified.The results were as follows:1.The best carbon and nitrogen source and inorganic salt of liquid culture medium for R.cyanoxantha were optimized by using of monofactorial and orthogonal test and basing the dry weight of mycelia as an evaluating indicator.The optimal carbon source was soluble starch,the nitrogen source was peptone and yeast extract,and the medium for R.cyanoxantha were composed of 3%soluble starch,0.3%peptone,0.3%yeast extract,0.05%KH₂PO₄.Basing on the optimal medium,the optimal culture conditions were optimized by using of orthogonal test,the results were as follows:27℃,initial pH 6.2,250 mL medium in a 500 mL flask,inoculation volume10 mL,rotation speed 150 r/min and fermentative time 4d.2.The mycelia of R.cyanoxantha were cultivated using liquid fermentation medium. Mycelia and cultured filtrates were extracted by petroleum ether(PE),ethyl acetate(EA), ethanol(Eth),respectively,and the different organic portions were prepared.The antioxidative assay showed culture filtrate and mycelial extracts obtained by EA,mycelial extracts obtained by Eth possessed higher antioxidative activities,and the activities of the extracts from EA were visibly higher than those obtained by PE.MTT assay suggested that culture filtrate extracts obtained by EA,mycelial extracts obtained by PE,EA and Eth could inhibit the proliferation of H446 cancer cells and exhibit higher antitumor activities.3.The components of mycelial extracts prepared by ethanol were analysed.Three fractions were obtained by chromatographic separation and named as Fractionâ… ,â…¡,â…¢, respectively.Fractionâ… was identified as 1-aza-bicyclo[4.2.0]oct-4-en-8-one by IR,FAB-MS, ¹H NMR,¹³C NMR,HSQC and HMBC methods.