Effects Of Plant Extracts On Growth Performance, Protein Synthesis And Liver Morphology In Carassius Auratus Gibelio

This experiment was conducted to evaluate the effect of three plant extracts(quillaja saponin,tea polyphenol,silymarin) and their blends on growth performance,potein synthesis and liver morphology in Carassius auratus gibelio.In trial 1,450 fishes(C.auratus gibelio autumn fingerlings) with an average body weight of 12.84±0.12g were randomly divided into 5 groups. Each group include 3 replicates,each replicate 30 fishes.The growth performance,blood biochemical indices,RNA/DNA and protein/RNA ratio of muscle and liver,nutritional composition of muscle,antioxidant indices and liver morphology in C.auratus gibelio autumn fingerlings were determined and analyzed after 56 feeding days while feeding with basal diets supplemented flavomycin(4mg/kg),quillaja saponin(150mg/kg),tea polyphenol(50mg/kg),silymarin(100mg/kg) respectively.The results showed that:①There were no any significant differences in growth performance within three plant extracts groups compared with the control group(p>0.05).②Quillaja saponins significantly increased albumin(ALB) content in plasma(P<0.05).Tea polyphenols significantly increased alkaline phosphatase(AKP) activity in plasma(p<0.05),significantly decreased blood urea nitrogen(BUN) content in plasma(p<0.05).Silymarin significantly improved AKP activity in plasma(P<0.05).③There were no any significant differences in RNA/DNA and protein/RNA ratio of muscle and liver within three plant extracts groups compared with the control group(p>0.05).④There were no any significant differences in nutritional composition of muscle within three plant extracts groups compared with the control group(p>0.05).⑤Silymarin significantly decreased maleic dialdehyde(MDA) content in plasma(P<0.05).Quillaja saponin and tea polyphenol significantly decreased maleic dialdehyde(MDA) content in liver(P<0.05).There were no any significant differences on superoxide dismutase(SOD) activity in plasma and liver in three plant extracts groups (p>0.05).⑥Three plant extracts don't damage liver and keep nomal liver morphology in C.auratus gibelio autumn fingerlings.In trial 2,the experiment was conducted to evaluate the effect of plant extracts and their blends on growth performance,potein synthesis and antioxidant indices of liver in C.auratus gibelio summer fingerlings by choose two plant extracts and their blends.700 fishes with an average body weight of 1.51±0.01g were randomly divided into 5 groups.Each group include 4 replicates,each replicate 35 fishes.The growth performance, RNA/DNA and protein/RNA ratio of muscle and liver,nutritional composition of muscle and antioxidant indices of liver in C.auratus gibelio summer fingerlings were determined after 56 feeding days while feeding with basal diets supplemented with compound plant extracts(1000mg/kg),quillaja saponin(150mg/kg),tea polyphenol(50mg/kg),quillaja saponin(150mg/kg) + tea polyphenol(50mg/kg) respectively.The results were as follows:①Compared with the control group,tea polyphenols significantly increased weight gain rate(WGR) and specific growth rate(SGR)(P<0.05).Compound plant extracts,tea polyphenols,quillaja saponin,tea polyphenol and their blends significantly decreased feed conversion rate(FCR)(P<0.05) and increased protein efficiency ratio(PER)(P<0.05).②Their blends with quillaja saponin and tea polyphenol group significantly increased total antioxidant capability(T-AOC) activity of liver compared with the tea polyphenols group(p<0.05) and decreased maleic dialdehyde(MDA) content of liver compared with the control group(P<0.05).The control group significantly increased glutathione peroxidase(GSH-Px) in liver activity compared with the quillaja saponins group and tea polyphenols group (p<0.05).③There were no any significant differences in RNA/DNA and protein/RNA ratio of muscle and liver in the test groups compared with the control group(p>0.05).④Compared with the control group,there were no any significant differences in nutritional composition of muscle in the test groups(p>0.05).In conclusion,There were no any significant differences in growth performance within three plant extracts groups compared with the control group in C.auratus gibelio autumn fingerlings.There were significant differences in growth performance within the tea polyphenol group and their blends with quillaja saponin and tea polyphenol group in C.auratus gibelio summer fingerlings.There were no any significant differences in potein synthesis with three plant extracts and their blends.Three plant extracts and their blends don't damage liver and keep nomal liver morphology in C.auratus gibelio.