Study On The Effects Of Fish Oil On Intestinal Mucosal Immune In Rats

In order to study the effects of fish oil on the intestinal mucosal immune of rats. SD rats were selected as experiment animals, the normal intestinal flora were observed by gradient dilution and cultivation method, and then the effects of fish oil on intestinal biological barrier in sweaned rats were investigated. The change of intestinal morphology index were measured using HE staining method, and then the effects of fish oil on intestinal mechanical barrier were explored. The effects of fish oil on inflammatory injury and SIgA secretion and expression were analyzed by immunohistochemical methods.The experimental results showed that: compared with control group, the average daily gain increased, the F/G decreased when added 0.5% of fish oil to basal diet of SD rats, thus fish oil can affect the performance of sweaned SD rats. Immune organs indices, the content of Enterobactes and Staphylococci and the rate of bacterial translocation all decreased obviously in experimental group. Fish oil increased muscular thickness, mucosal thickness, length and width of villus in vary degree. The disease activity index, colon mucosal and colon tissue injury reduced, while the levels of IgA,IgG, IgM in serum and colon tissue increased, the expression level of SIgA in colon improved after feeding fish oil.Based on these data, fish oil could enhance intestinal immunity through improving the intestinal biolo -gical barrier, mechanical barrier and immune barrier.