The Dissection On Pathogenic Mechanism Of Arthrinium Phaeospermum To Bambusa Pervariabilis×Dendrocalamopisis Grandis Hybrid Bamboo

This experiment with Arthrinium phaeospermum (corda) MBEllis and Bambusapervariabilis×Dendrocalamopisis grandis hybrid bamboo as the test material, selected abest culture conditions of toxin-producing, and investigated the physical-chemicalpeculiarity of Arthrinium phaeospermum crude toxin, extraction and purification asubstance of the Arthrinium phaeospermum toxin. The study also with hybrid bamboo bytreatment with three concentrations of pure toxin as the trial materials to determination ofphysiology and biochemistry index.All studies provides a theoretical basis about thepathogenic research of Arthrinium phaeospermum toxin on hybrid bamboo. In additionuseing the electron microscope technique to study the changes of hybrid bamboo leavesby treated with three concentrations of pure toxin. Main research results are as follows:1. Useing hybrid bamboo biological detection for guidance, selected Arthriniumphaeospermum a most toxin-producing bacteria medium that is Fries and the bestculture conditions of toxin-producing is: 25℃, 12d, pH8.0, oxygen capacity is morethan 100ml, no significant effects of light conditions.2.Crude toxin of Arthrinium phaeospermum by preparated in the best training with Frise medium under the best condition of the training, by testing the crude toxin with strong thermal stability; of pH-sensitive, prone to alkaline substances impact; molecular weight of toxin active ingredient is less than 8000 molecular weight; biological active protein molecules in metabolites below small molecule toxins, Arthrinium phaeospermum crude toxin of bioactive components are mostly larger polar substances.3. By thin-layer chromatography and column chromatography, separation and purification the relatively pure substance, then through nuclear magnetic resonance, infrared spectroscopy to detecta active toxin monomer is a polysaccharide with a chemical formula C₁₁H₁₈O₂.4. In hybrid bamboo can tolerate the concentration range of toxins, free proline, soluble protein content increased by a certain extent, but more than the toxin concentration range of cracking, etc may result other physiological and biochemical changes, which led to Organization of the original loss of function resulted in a sharp decline in free proline content; PAL, SOD, POD activity decreased after the first enhancement; soluble sugar content fluctuated changes, but the overall trend is downward.5. Water treated bamboo tablet hybrid, and its ultrastructure of mesophyll cells are more well-defined, dense cytoplasm can be seen neatly arranged lamellar structure of chloroplasts and photosynthesis particles hobby. Toxin and treated with leaf cells, diffuse cytoplasmic. There are varying degrees of damage, destruction of chloroplast structure, with the increase in toxin concentration, the structure of the chloroplast lamellar and particles of photosynthesis hobby reduced, while the degree of damage of lamellar structure is gradually increasing.