| To establish a stable and efficient micro-propagation system for Scindapsus aureus,the young leaves,used as explants,were cultured in vitro and plants were regenerated via organogenesis in this study.The affecting factors were analyzed and discussed,including plant growth regulators,basic media,carbon sources,kinds of explants and concentrations of AgNO3 and GA3.The results were as follows.(1) The best way of explant sterilization was 0.1%HgCl2 for 6 minutes.(2) The concentrations of TDZ and auxin had significant effect on the callus induction from the leaf,and the kinds of auxin had extremely significant effect on the callus induction from the leaf,the suitable concentration of TDZ for the callus induction was 0.1-0.5 mg·L-1,and the suitable concentration of auxin was 0.1 mg·L-1-0.5 mg·L-1,the suitable auxin was NAA,the medium MS+0.2mg·L-1TDZ +0.5mg·L-1NAA was suitable for the callus induction,resulting in 97.62%of callus induction rate.(3) The basic medium had significant effect on the callus induction from the leaf;the suitable medium was MS for the callus induction.(4) The carbon source had significant effect on the callus induction from the leaf,and the sucrose was suitable carbon source for the callus induction.(5) Dark culture had no significant effect on the callus induction from the leaf.(6) The kinds of explants had significant effect on the callus induction from the leaf; the suitable explants were leaf(with midrib) and petiole(petiole base) for the callus induction. (7) The kinds of auxin had no significant effect on the shoot induction,and the kinds and concentrations of cytokinin had significant effect on the shoot induction;the suitable cytokinin was 6-BA for the shoot induction and its suitable concentration was1.0-2.0mg·L-1, the medium MS+0.1mg·L-1 NAA+1.0mg·L-1 6-BA and MS+0.5 mg·L-1NAA+2.0 mg·L-1 6-BA was suitable for the shoot induction.(8) The kinds of cytokinin had extremely significant effect on the multiplication rate of buds,and its concentration had significant effect,whereas the concentration of auxin had no significant effect on the multiplication rate of buds;BA was the most suitable for the multiplication of buds of the three kinds of CTK,BA,KT and TDZ,and its suitable concentration was 2.0mg·L-1;the medium MS+2.0mg·L-1 6-BA+0.1mg·L-1 NAA was suitable for the multiplication of buds.(9) AgNO3 could stimulate plant regeneration,the suitable concentration for multiplication of buds was 3.0 mg·L-1,resulting in a bud multiplication rate of 2.05, and without the formation of deformed buds.(10) GA3 could stimulate plant regeneration,and with the increase of GA3 concentration,the proliferation of adventitious buds increased at first and decreased afterwards;the suitable concentration was 3.0mg·L-1 for the bud multiplication at which the average proliferation rate was 1.71.(11) The concentration of BA had no significant effect on plantlet culture,the type and concentration of auxin had significant effect;the medium MS+1.0 mg·L-1 6-BA+0.5 mg·L-1 IBA was suitable for the growth of plantlets,and the medium MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 IAA was suitable for occurrence of new leaves of plantlets.(12) Roots could be easily induced,but the quality of the resultant roots on different media was obviously different;the best quality of roots,with several healthy branches and no callus,could be induced on the optimal rooting medium 1/2MS+0.1mg·L-1 NAA +0.2mg·L-1 IBA,giving rise to a rooting rate of 100%and an average root number of 5.74. |
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