Lily Distant Hybridization And Seed Characteristics

Lily (Lilium)was rich on germplasm resource, long history of cultivation, various in flower type, showy in flower colors and widely planting, it can be used for cut flowers decoration and yard virescence. Furthermore, bulbs also were food and medicinal value. Lily is beautiful, refined, elegant, aromatic and pleasant. It has been the symbol of pure, bright, freedom and happiness. Lily is famous for the king of bulbous flower.Our country is the hometown of Lily, but Lily breeding is very late and rarely independent intellectual property rights lily varieties. In most areas of northern China, the climate is cold, which makes the vast majority of the lilies from the Netherlands introduced species should not show their caste. Therefore, natural selection new varieties of lily can grow at the climate and environment in northern of China, were great practical significance about the current breeding of lily.In this study, used the hybrid lilies as cultivars parental samples belonged to the Asian lilies, Oriental lilies, Lilium longiflorum three lines and three categories monocrotaline, four main categories, total 14 species resource, via the biological characteristics species research of these 14 Lily species, and mensurated their pollen viability, were determined in light of relationships, cold hardiness, flower color, aromatic, green, such as length of breeding goal, the 14 varieties of hybrid combination of lily of the parent matching, a total design, including distant hybridization, Intraspecific hybridization and the hybrid combination of self-112, with an average for each combination of pollination of hybrid 107, a total of 12,000 flowers pollinated flowers. Hybridization using conventional direct pollination, cutting stigma pollination, pollination, and the pros and cons of hybrid decoy different pollination methods, such as pollination, fertilization after pollination barriers to cause abortion after the 53 combinations of embryo rescue, the hybrid seedlings; At the same time, access to the 59 kinds of hybrid seed dormancy and germination characteristics of the study, seven kinds of media and the use of treatment methods such as peeling, seeding with conventional control, distant hybridization of lily seeds excavated from the type of seedling germination rate and rate of the study; Finally study on the F1 generation growth and ornamental characters of some Asian Lily female parent.The results were as follows:1. Via observed these 14 Lily species of the biological characteristics, the Asian Lily rich color of the flower type, cold and strong, short growth cycle, particularly in 'Pollyanna' and 'Elite' good performance. Oriental lily, and good petal texture, aromatic and pleasant, especially the 'Sorbonne' was good breeding potential; 'L.lancifolium' and ' L. dahurcum' cold, drought and strong, suitable material, as resistance breeding. 2. Pollen viability was determined by germination of law, through the Duncan multiple comparison results showed that: apart from 'L.formolongi', the other 13 varieties of lily pollen germination rate of the difference is not significant, the average pollen germination rate in more than 50%, the highest the 'Sorbonne' pollen germination rate reached 93.62%, both can be used as male parent of hybrid materials.3. Lily distant hybridization results showed that: Asian lily cultivars as female parent in hybrid combinations, ovary enlargement rate and the average number of high seed; and Oriental lily cultivars as female parent in hybrid combinations, ovary enlargement of the small, there was The enlargement does not, did not receive hybrid seed. 'L.lancifolium' as the female parent of the hybrid combinations, particularly 'Pollyanna' and 'L.lancifolium' were most hybrid seeds. Swollen ovary at around from 1.8 to 2.2, the highest seed-setting rate, access to good seed plumpness.4. Lily distant hybridization used different hybridization methods, the majority of hybrid combinations with conventional direct hybridization method Pollination was relatively good. Decoy pollination methods applied to between some Oriental lilies and Asia Lilium hybridization. To some extent, cutting stigma pollination methods can solve distant hybridization before fertilization barriers, to be combined with embryo rescue methods of cross-breeding.5. Hybrid seed dormancy and germination studies showed that: cold stratification 40d, 70d, 90d and 120d after 400mg / L GA3 better able to deal with hybrid seeds to break dormancy, cold stratification to one of 90d, 400mg / L GA3 treatment was most effective , seed germination rate than the single 400mg / L GA3 soaking treatment improves 7.78%.6. The research of hybrid seed tissue culture method, seed sterilization method was sterilization in 70% ethanol for 60 seconds, and then 2% NaCl03 Sterilization 7 ~ 15 minutes; The best medium was MS + NAA0.1mg / L +6- BA0.5mg / L + sucrose 30g / L for the hybrid seed germination7. Asian Lily for parents to obtain the F1 generation, flower type and color occurred variation. The flower type changed from the pro-bowl type into petals. The color variation shown at the flower yellow, petals without spots, and the color of the parents were white and orange.