Effects Of Phosphate Fertilize On Bulbs Of Tissue Culture Seedings Of Lily Tiber

Taking the Oriental hybrid lily Tiber tissue culture seedling as materials, study of rooting and forming of bulblets in tube and fertilization technology of phosphorus was conducted. This experiment studies the effects of different concentrations of PP333, NAA and sugar on rooting and bulblets formation of Lily plant. In the greenhouse, potted conditions, the effects of different total of applied P, way of applied P and interactive between them on growth and physiological of the Lily tissue culture seedling was studied, in the purpose of finding high quality cultivation techniques in bulbs of Lily tissue culture seedling and providing technical reference for oriental lily seed of large-scale localized production. Five levers of phosphorus fertilization supply was designed:0kg/667m2(CK), 11.04kg/667m2 (A1),22.08kg/667m2 (A2),33.12kg/667m2 (A3) and 44.16kg/667m2 (A4); Phosphorus fertilization methods were designed into three levers:B1 (100% as basic fertilizer), B2(60% as basic fertilize and 40% average for four times as top-dressing and hole applied); B3(60% as basic fertilize 40% average for four times as spray on leaves). The chief results are showed as follows:1. PP333 favored roots-taking and bulblets forming of the Lily tissue culture seedling in the tube. The best concentration was 2.5mg/L; The NAA benefited rooting but had not obvious effect on bulblets forming, the better concentration was 0.5 mg/L; The sugar benefited rooting and bulblets formation, the better concentration was 60g/L.2. When Phosphorus fertilizer 100% was used as basic fertilizer (B1 treatment), A2 treatment (22.08kg/667m2) had the highest Lily plant, the biggest stem diameter, the most number of leaf and the highest chlorophyl contents of Lily leaf. These indicators in treatment A2 were higher respectively 45.6%,44.4%,29.3%,20.2% than those of CK. In treatment A3(33.12kg/667m2),fresh weight of root, perimeter and fresh weight of bulb and the content of starch, soluble sugar, and protein was the highest. The content of total phosphorus increased as supply increasing of phosphorus fertilization.3. In the experiment of the effect of different application of Phosphorus fertilizer, when the application amount of Phosphorus fertilizer was A2 treatment (22.08kg/667m2), the B3 treatment (60% as basic fertilize 40% average for four times as spray on leaves) was better than other treatments in height of Lily plant, number of leaf, stem diameter, fresh weight of root, perimeter and fresh weight of bulb and the content of starch, soluble sugar and protein. The chlorophyl content of Lily leaf of treatment B1 (100% as basic fertilizer) was the highest.4. When different treatments of interaction between the supply amount of phosphorus fertilization and supply methods, contrasting all indexes of different treatments, the A2B3 treatment (22.08 kg/667m2 and 60% as basic fertilize 40% average for four times as spray on leaves) was the best treatment in this experiment.