Tissue Culture To Future Generations Of Hybrid Lily

This test utilizes the generation of F1scales getting from the seeds and embryos of the hybrid of Asia series of lily varieties:’Prato’,’Pollyanna’,’Brussels’with the east of lily varieties series:’Samur’; Wild lily:L.davidi,. We study the research for five stages, including adventitious bud inducted by scales, multiplication of adventitious bud, in vitro shoot and swollen bulbs generation, producing root and transplanting to soil. The main research results are as follows:The optimal medium for inducing offspring mixed scales vitro shoot of the hybrid’Prato’with’Brussels’,’Prato’and’Pollyanna’isMS+6-BA1.0mg/L+NAA0.05mg/L,the differentiation rate is100%, and the differentiation coefficient is3.75; The optimal culture medium for the proliferation vitro shoot is MS+6-BA2.0mg/L+NAA0.01mg/L, on average, the proliferation bud number of is5.64; The best culture medium for generation bulbs induced by vitro shoot is MS+6-BA0.01mg/L+NAA0.5mg/L, the bulb average diameter is1.12cm mostly, bulb increase7.5times as much as the control, the inducting bulb rate is100%; Root induction medium is MS+6-BA0.01mg/L+NAA0.5mg/L, the average root number is20.25, the inducting rooting rate is100%.The optimal medium for inducing’Prato’and’Pollyanna’hybrid offspring scales vitro shoot is MS+6-BA1.0mg/L+NAA0.05mg/L, differentiation rate is100%, and the differentiation coefficient is3.57; The optimal culture medium for proliferating vitro shoot is MS+6-BA2.0mg/L+NAA0.05mg/L, the average bud proliferation number is4.92; The best culture medium for generation bulbs induced by vitro shoot is MS+6-BA0.01mg/L+NAA1.0mg/L, average bulb diameter is0.88cm mostly, the bulb increases alomst3.8times, the inducing bulb rate is100%; The optimal root induction hormone medium is MS+6-BA0.01mg/L+NAA1.0mg/L, the average root number is16.88, inducing rooting rate of100%.The optimal medium for inducing’Pollyanna’natural pollination hybrid offspring scales vitro shoot is MS+6-BA0.5mg/L+NAA0.10mg/L, the differentiation rate is93%, and the differentiation coefficient is2.85; The optimal culture medium for proliferating vitro shoot is MS+6-BA2.5mg/L+NAA0.01mg/L, on average, the proliferation bud number is4.56; The best culture medium for generation bulbs induced by vitro shoot is MS+AC1.0mg/L, the average bulb diameter is0.81cm, the bulb increase about3.6time than the control, the inducing the bulb rate is100%; The optimal root induction hormone medium is MS+6-BA0.01mg/L+NAA0.5mg/L, the average root number is 9.76, inducing rooting rate of100%.The optimal medium for inducing’Pollyanna’natural pollination hybrid offspring scales vitro shoot is MS+6-BA1.5mg/L+NAA0.01mg/L, the differentiation rate is85%, and the differentiation coefficient is3.47; The optimal culture medium for proliferating vitro shoot is MS+6-BA1.0mg/L+NAA0.01mg/L, on average, the proliferation number is4.55; The optimal culture medium for proliferating vitro shoot is2MS, the bulb average diameter is0.63cm, bulb increases4.4times, the bulb forming rate is84%; The optimal root induction hormone medium is MS+6-BA0.01mg/L+NAA0.5mg/L, the average root number is25.73, the inducing rooting rate is100%.The optimal medium for inducing’Prato’head and L.davidi hybrid offspring scales vitro shoot is MS+6-BA1.0mg/L+NAA0.05mg/L, differentiation rate is85%, and the differentiation coefficient is2.12; The optimal culture medium for proliferating vitro shoot is MS+6-BA2.5mg/L+NAA0.05mg/L, on average, the proliferation number is2.95; The optimal culture medium for proliferating vitro shoot is2MS, the bulb average diameter is MS, the bulb average diameter is0.86cm, bulb increases4.2times, the bulb forming rate is100%; The optimal root induction hormone medium is MS+NAA0.5mg/L+AC1.0mg/L, the average root number is7.55, the inducing rooting rate is100%.This study explores the hormone types and the concentration influence on the five hybrid lily tube seedlings growth and the bulbs, we choose five optimum medium for hybrid lily growth and bulb swollen. Genetically modified lily can provide good breeding for the large scale production. The present study is still at the stage of tissue culture, the next step can focus on the five types of hybrid lily patterns, design, color and fragrance sex, cold hardiness.