Extraction And Application Study Of DNJ From Mulberry

The study mainly talks about the utilization of mulberry branches, as a major by-products produced in the sericulture. According to the report, the production of cutting branches can reach to500-800kg/mu for one year and a total output of 2.5×105-4×105 tons was acquired from mulberry gardens of 500 thousand mu in Anhui province. There are 0.1%-0.2% of alkaloids 1-deoxynojirimycins in mulberry branch which used as efficient and expensive diabetes for disease treatment. According to extraction rate of 0.1% DNJ from the tissues, these branches could produce 1000-8000 tons of 10%-30% of DNJ crude extracts and bring out several millions RMB income. In this experiment, extract method and hypoglycemic effect of DNJ from mulberry is analyzed and this study may provide theoretical reference for the further utilization of cutting mulberry branches in summer.After being extracted by distilled water, DNJ was purified by iron-exchange column chromatography and its content distributed in different tissues was determined by UV spectrophotometric. The results show DNJ content in various parts of mulberry was different: higher levels of DNJ in buds, branches and barks before germinate of mulberry were found, 0.15% in the branches and DNJ contents in barks and buds were followed. However, lower DNJ concentration was determined in leaves and roots of mulberry and was lowest as 0.03% in barks after germinating. These results lay the base for the extraction of DNJ from different tissues of mulberry.Taken the branches as experimental material, choose the various conditions such as solvents, time and temperature to analyze the technical parameters. By comparison of different extraction conditions, 80% of ethanol, 60℃and 2 hours treament was optimum and the DNJ extraction rate reaches 0.176% ,while extracted with ethanol is better than distilled water. As far as the infuencing factors on DNJ extraction was concerned, the temperature was more than solvent and time.We also found that with the time increases, the DNJ content in the deposited mulberry branches decreases: 0.148 % for half a year, 0.139% for one year and 0.126% for one and half a year,respectively.To investigate the blood-glucose lowering effect of DNJ from mulbeery, the animal experiment was performed using hyperglycemic mices model. The results indicates the blood sugar of mice decreases by 7.63%,15.72% and 18.41% after being gavaged with equivalent extract, double amount of extract and metformin, respectively. In addition, blood sugar value of treated mice after 4 weeks decreases with different deposited time of mulberry branches, namely, 15.83% and 6.81% for half a year and one year respectively.