Technique Of Tissue Culture And Rapid Propagation Of Rhododendron Chrysanthum Pall And Analysis Of Effective Constituent

The distribution of Rhododendron chrysanthum Pall is very narrow in China,it just Distributes in a certain region of Chang bai mountain. Because of its high ornamental value and medicinal value, great development potential.In order to increase the number of Rhododendron chrysanthum Pall populations of domesticated and landscaping,to achieve its development and application of medicinal value, In this study, with the seeds of Rhododendron chrysanthum Pall as test material, to study the technique of tissue culture and rapid propagation; the test adopted HPLC and Ultraviolet Spectrophotometry separately to test main medicinal component Rutin and Tannins of Tissue Culture Plantlet and wild seedling of Rhododendron chrysanthum Pall Chang bai mountain. Its findings were as follows:1 The embryo of Rhododendron chrysanthum Pall best disinfectant combinations as follows:With 75%alcohol of disinfection for 30s,0.1%mercuric chloride sterilization for 3min; Best of initial medium as follows Read+GA32.00 mg-L" germination rate reached 8.8%;2 The best medium for subculture of Rhododendron chrysanthum Pall was modified MS+ZT1.0mg·L-1+IBA0.5 mg·L-1, multiplier effect is the best, culture after 40 days multiplication and increase the height reached 5.04 and 1.56cm;3 The more suitable for medium for leaves induction callus was modified MS+TDZ 1.0 mg·L-1+2,4-D 0.3 mg·L-1+dark culture for 15days, the callus rate reached 100%; The more suitable for medium for callus differentiation of Rh. chrysanthum Pall was modified MS+TDZ 1.5 mg·L-1+NAA0.05 mg·L-1, the differentiation rate reached 95%;4 The more suitable for medium for rooting in bottle of Rhododendron chrysanthum Pall was 1/4 modified MS+IBA1.5mg·L-1+sucrose20g·L-1, rooting rate reached 100%.5 The best method for acclimatization and transplants of tissue culture seedling of Rhododendron chrysanthum Pall was first to exercise 10 days in riversand,then transplanting in the matrix of humus soil:turfy soil:riversand=2:1:1, survival rate reached 90%.6 Overwintering modle for safety of Rhododendron chrysanthum Pall was the whole plant was covered with soil,in order to prevent low temperature injury.7 The component Ruin and Tannins in wild seedling is higher than Tissue Culture Plantlet.