Study On The Culture Characteristics Of Mycelium And Artificial Domestication Of Lepista Nuda

Lepista nuda belong to the Basidiomycotina,Hymenomycotina,Agricales, Tricholomataceae,Lepista and is also named Hua lianmo,Luo koumo,Zi jingmo.It is a new and rare medicinal fungus.It has extensive market apply foreground for value of medicinal and cancer prevention healthiness.The research summary of taxonomic status and value of medicinal and apply foreground of Lepista nuda.This table introduce the present research status at home and abroad of mycelium growth characteristic and domestication.So the paper researched of nutrition characteristic and environment condition for mycelia growth,filter of nourishment material formula and domestication of Lepista nuda.The result showed that:The pure mycelium isolated from the fruit body tissue of Lepista nuda was used as tested material.This paper studied on the optimum carbonic sources,nitrogenous sources,inorganic salts and the best combination on the growth rate of Lepista nuda was studied by single factor and orthogonal experiment. The result showed that:sucrose is the most optimal carbonic source,white sugar and glucose is the second;yeast extract is the most optimal nitrogenous source,yellow soybean powder is the second;MgSO4 is the most optimal inorganic salt,CaSO4 is the second.The orthogonal experimeng and ANOVA showed that the optimum combination is sucrose 25g·L-1,yeast extract 2.0g-L-1,MgSO4 0.5g-L-1. The primary and secondary order of effect mycelium growth factor is C→B→A.The best tempertature for mycelium growth of Lepista nuda is 25℃.The suitable pH extension between 6 and 7.The growth rate of everyday and dry weight of Lepista nuda mycelial are the best of all,and the mycelia is dense and thick.The feasible concertration of CO2 is favorable for mycelium growth.The mycelia is the best under the containing water of culture material on 65% and 60%.The mycelia is not good under the containing water of culture material higher or lower.The best mother culture for mycelia growth of Lepista nuda was improved yellow soybean powder culture medium(yellow soybean powder 40g,peptone 2g,glucose 20g,agar20g,add water to 1000ml).The suitable original spawn substrates is boxwood dust 73%,straw 5%,yellow soybean powder 15%,white sugar 3%, CaCO32%, KH2PO40.5%,lime 1%,cast 0.5%.The best cultispecies substrates is dry dung 55%,straw 36%,beancake powder 5%,CaCO32%,KH2PO40.5%,lime1%,cast 0.5％.