Font Size: a A A

The Study Of Lepista Sordida (Fr) Sing Strains

Posted on:2010-07-05Degree:MasterType:Thesis
Country:ChinaCandidate:W D QuFull Text:PDF
GTID:2143360275985197Subject:Vegetable science
Abstract/Summary:PDF Full Text Request
Lepista sordida (Fr) Sing is a valuable specie which is famous for its deliciousness and medical effectiveness. Until now there is not any report about its acclimatization. In this study, we did a systematical research from its biological characteristics to economical character. The results are as follows:First, the research on biological characteristics: the optimum conditions for the growth of mycelium are: temperature at 25℃,pH at 6-7; cornmeal and flour as the carbon source; bean flour as nitrogen source; C/N ratio as 15/1; VB1 as vitamin and we also find that the mycelium is nothing sensitive to light..Second, RAPD molecular marker technology was used to do the cluster analysis from which we could see that the genetic distance of the strains tested is 0-0.46. Tested strains number 2 and number 8 are comparatively alien to other strains respectively.Third, spore germination factors research: the result showed that the optimum temperature for spore germination is 25℃, the optimum concentration of spore is 6-20, and the heated temperature and heated time to the spore solution are 40℃, 30 min.Forth, culture experiment: we find that strains tested are all aeromicrobe. Tested strain number 8 has shorter growth cycle, bluer fruiting body, and highest yield compared with other strains.Fifth, mensurating the content of enzyme in different phrase, we can tell that the activity of enzyme is closely related with the phrase it was in. It was usually high when the mycelium is growing mature.Sixth, nutritious elements research: the strains tested were rich in nutritious elements, and have a full serial of amino acid. Among the strains, strains of number 2 has the highest level of lysine, and also the essential amino acid.
Keywords/Search Tags:Lepista sordida (Fr) Sing, domestication, biological characteristics, economical character, hereditary property, resistibility, nutritious elements
PDF Full Text Request
Related items