Isolation And Identification Of The Pathogen Of Cow Endometritis And The Preparation Of Uterus Suppository

In order to treat the cow endometritis, 20 endometritis cows in 3 large farms of XingTai, Hebei province were selected, their uterus secretions were studied by microscopic examination, isolation and culture of pathogens, biochemical identification etc. Under this foundation, the rabbit endometritis pathological model was made by perfusion of pathogenic E. coli, staphylococcus, streptococcus, Salmonella, Bacillus cereus and other mixed bacilli to the uterus. The schemes of the suppository preparation were optimized by orthogonal test, the color, insoluble substance, melt time were used as standard to prepare the traditional Chinese medicine suppository, the compound iodine suppository and the biological suppository. The suppositories were put to the uterus by 0.2g/kgï¹’bw in 1st, 3rd, 5th days respectively, the clinical symptoms, uterine shape, pathological changes of endometrium, SOD, MDA, GSH-Px in sera, and other biochemical parameters were observed. The suppositories were put into the uterus of 30 endometritis cows respectively. Clinical symptoms were observed, and serum SOD, MDA, GSH-Px were examined in 0d, 2d, 4d, 6d, 8d treatment.The results showed that 6 pathogens were isolated in the uterus secretions, they were Staphylococcus aureus(8, 34.78%), Staphylococcus epidermidis(6, 26.09%), Streptococcus(2, 8.70%), Salmonella(2, 8.70%), E coli(4, 17.40%), Bacillus cereus(1, 4.35%) respectively. The traditional Chinese medicine suppository, the compound iodine suppository and the biological suppository were fit the standard of the pharmacopoeia. Compared with the indexes before using the suppositories, the results of rabbit endometritis pathological model after using the suppositories showed that the vulva edema and vaginal congestion subsided gradually, the uterine secretions reduced gradually. The uterine cervix and horn swelled slightly, there was a small amount of clear secretions in the uterus. The endometrium was more integrity, the structure of epithelial cell was normal. SOD and GSH-Px activity in sera were increased significantly (P<0.05) by 4 days treatment of compound iodine suppository. Serum SOD activity was increased significantly (P<0.05), serum GSH-Px activity was increased very significantly (P <0.01), serum MDA content was decreased very significantly (P <0.01) by 6 days treatment of compound iodine suppository. There were no significant change of the clinical symptoms, the serum SOD, GSH-Px and MDA in the traditional Chinese medicine suppository and biological suppository groups. The results on cow endometritis after using the suppositories showed that the uterine secretions of cows were obviously decreased. In 6d treatment, serum SOD activity increased significantly(P<0.01), serum GSH-Px activity increased significantly(P<0.05), and serum MDA content decreased significantly (P<0.01) in the compound iodine suppository groups. In 8d treatment, serum SOD activity increased significantly (P<0.05), serum GSH-Px activity increased significantly(P<0.01), and serum MDA content decreased significantly(P<0.01) in the biological suppository groups. While serum GSH-Px activity increased significantly (P<0.05) and serum MDA content decreased significantly (P<0.01) in the traditional Chinese medicine suppository groups. The compound iodine suppository was optimized in the suppositories for treatment of endometritis.