The Karyotype Study And The AFLP Analysis Of Genetic Relationships Of Some Lycoris Species In China

Lycoris is a genus of more than 20 flowering plant species in the family Amaryllidaceae, Monocotyledoneae. They are native to eastern and southern Asia in countries such as China, Japan, Korea, Burma, Nepal, and Indonesia. China has 14 species and 2 varities of Lycoris, most of which are found south of the Yangtze River.We collected samples of 20 materials of Lycoris from Jiangsu, Zhejiang, and Henan. To gleam some insight into the genetic diversity and phylogenetic relationships of these Lycoris species and varities, we conducted karyotype analysis on the 13 materials and obtained silver-stained AFLP fingerprints of the 20 materials. UPGMA clustering analysis was used to analyze the AFLP fingerprint profiles.We observed six chromosome classes (m, sm, st, t, T, and B) and four karyotypes (4A, 3B, 2B, and 3C). Some improvements were made to the existing Lycoris genomic DNA extraction technique. Using six pairs of AFLP primers selected from 33 candidate pairs, we obtained 330 amplified DNA bands, of which 320 were polymorphic. The species and varities used in the AFLP fingerprinting study have interspecific genetic similarity (GS) coefficients ranging from 0.38 to 0.89. Sympatric species appear to be more closely related than allopatric species. the most materials have yellow flowers were together.Both karyotype analysis and AFLP technique were efficient in generating more accurate information on the genetic diversity and relationship of Lycoris.