| The present study aimed to study the effects of partial or total fishmeal substitution by animal protein blend (APB, poultry by-product meal∶beef meat and bone meal∶spray dried blood meal∶hydrolyzed feather meal = 40∶35∶20∶5) or fermented soybean meal (FSM) on growth performance, flesh quality, nitrogen and phosphorus metabolism under ideal digestible amino acid profile (IDAA) in Japanese sea bass (Lateolabrax japonicus). 4 trails including in this study were conducted in recycling water system (volume, 0.3m3) of National Aqua-feed Safety Assessment Station, Feed Research Institute, CAAS. And the results were respectively summarized as follows:1. This growth trail was conducted to study the effect of 0%,50%,75%,100% dietary low temperature steam-dried fish meal (LT-FM)substitution by APB with or without crystallized Lys,Met,Thr supplementations on growth and body composition in Japanese sea bass (13.2±0.05 g). 8 weeks later, results showed that: growth performance of sea bass stepped down with increasingly replacing level; fish fed the control diet had significantly (P<0.05) higher SR,FR,FBW,SGR,WGR,PER,feed efficiency than those fed diets containing APB; sea bass fed diets with supplementations of crystallized amino acids showed remarkably higher FR,FBW,SGR,WGR,PER,feed efficiency and crude protein, crude lipid, gross energy but lower ash and moisture of than their counterparts in which LT-FM were replaced directly (P<0.05). Results demonstrate that 1) no more than 50% of LT-FM can be replaced by APB in diet of Japanese sea bass (13.2±0.05 g); 2) Supplementations of crystallized amino acids improve potential of APB replacing dietary LT-FM of sea bass; 3) crystallized amino acids can be efficiently utilized by sea bass.2. This growth trail was conducted to study the effect of 0%,50%,75%,100% dietary LT-FM substitution with crystallized Lys,Met,Thr supplementations by APB on growth and body composition at 2 digestible protein levels (DPL, 38.5% vs 35.5%) in Japanese sea bass (13.25 g±0.05 g). 8 weeks later, results showed that: 1) fish fed 0% diet had significantly (P<0.05) higher SR,FR,FBW,SGR,WGR,PPV,EPV,feed efficiency than those fed diets containing APB; 2) fish fed diets with low DPL diets displayed remarkably (P<0.05) lower FBW, WGR, ERE feed efficiency than their counterparts in high DPL group; however, no differences were found on CF,HSI,VSI and whole body composition between the 2 DPL. Results demonstrate that under IDEAAP 1) high protein level could expend application of APB in diet of Japanese sea bass (13.25 g±0.05 g); 2) no more than 50% of LT-FM can be replaced by APB in diet of Japanese sea bass (13.2±0.05 g).3. This growth trail was conducted to study the effect of 0%,20%,40%,60%,80% dietary Peru fish meal (PFM)substitution by APB with Lys,Met,Thr supplementations on growth and flesh quality of Japanese sea bass (76.3g±0.2g). Another diet containing 50% local fish meal (LFM) formulated according to PFM was added to test how different qualities of FM affected fish growth performance. 8 weeks later, results showed that: growth performance was decreased with higher APB inclusion level, and APB60 and APB80 showed significant lower SR,FBW,SGR,WGR,PPV,EPV and nutritional value of muscle than the other groups (p<0.05). Fish of LFM group showed significant poor FR and cooked muscle texture values than those of PFM, but did not show remarkable difference in growth profile. No differences of plasma ALT and AST activities were found between the groups. Results demonstrate that: 1) APB could reduce dietary PFM from 400mg/kg to 160mg/kg without depressing growth or affecting flesh quality of Japanese sea bass; 2) more than 240mg/kg inclusion of APB would induce poor growth and nutritional value of sea bass. 3) poor quality of fishmeal would reduce FR and flesh quality of bass, PFM is remarkablely better than LFM, 40% dietary PFM had the same breeding effect of 50% dietary PFM.4. This growth trail was conducted to study the effect of 0%,25%,50%,75%dietary LT-FM substitution by FSM with Lys,Met,Thr supplementations on growth,flesh quality and nitrogen and phosphorus metabolism of Japanese sea bass (13.25 g±0.05 g). 8 weeks later, results showed that: SR,FR,FBW,SGR,WGR,PER,EPV,feed efficiency,body composition and hematological parameters were not different (P>0.05) between WFM and FSM25 group, but both of them performed better than the other groups (P<0.05). Fish fed diet FSM75 showed too low growth performance in first 8w and did not continue the last 8w growth trial for this group. 16 weeks later, results showed that: Fish fed FSM50 diet showed compensatory growth in the latter 8w, which showed significant higher FR, SGR, WGR and PRR than those of WFM and FSM25 groups during this period, but still showed lower PPV,EPV and feed efficiency than those of fish fed diets WFM and FSM25 (P<0.05). Fish of FSM50 group showed lowest flesh-hardness, flesh-gumminess, flesh-chewiness. Results demonstrate that: 1) FSM fermented by distiller's yeast could replace no less than 25% of dietary LT-FM in sea bass, without influencing its growth, feed utilization and flesh quality; 2) in the long run, present FSM could replace 50% of dietary WFM in sea bass with good growth performance and feed utilization through compensatory growth, but with lower flesh quality and higher nitrogen excretion.
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