Study On Seedborne Fungi Of Sainfoin(Onobrychis Viciaefolia Scop)

Sainfoin (Onobrychis viciaefolia Scop) is an outstanding perennial legume with a reputation as a "grass queen". It is playing an increasingly important role in the ecological construction and agricultural production of western China. Diseases limit the production and application of sainfoin. Seeds are an important site for pathogenic infection. In this paper, water agar was used to isolate and identify fungal species in three different varieties of sainfoin across 10 different bean-producing plots. Species were identified on the basis of seed inoculation with spore suspension method. In addition, the effects of fungi on the quality and growth of seedlings was examined,:1. From the three different species of seeds,20 genera and 25 species of fungi were isolated and identified using water agar. The percentage of fungi isolated from Disinfection of contaminated surfaces was significantly lower than infection. Among those fungi species isolated, Alternaria alternata, Alternaria sp., Aspergillus niger, Rhizopus stolonifer, Alternaria, Penicillium sp., Phoma sp., Ascochyta onobrychidis Helminthosporium, Curvularia sp., Cladosporium sp., Aspergillus flavus, Fusarium moniliforme, Fusarium oxysporum, were common fungi in sainfoin seeds. Phoma sp., Trichoderma sp., Cladosporium sp., Colletotrichum sp., were the 5 species isolated for the first time in China.2. Seed infection percentage and germination rate were found to have a significant negative correlation. Removing the seed pod skin can improve the germination rate of sainfoin. Seed infection rate and the moisture of the producing area have a significant positive correlation, where as a significant negative correlation exists between infection rate and the elevation of the producing area.3. After dissection of seed and cultured on water agar method the results informed that the isolation rate of fungi species with external inward from seeds in turn. The highest concentration of fungal species were found to exist within the seed pod in the skin, followed by the seed coat, cotyledon and embryo, the number of fungi species isolated from each part was 16 species, followed by 11 species, and 6 species respectively.4. For the trial of 16 species of fungi, each can significantly lower the quality of sainfoin seed quality and reduce seed vigor index and seedling growth of health effects. Fusarium oxysporum and Fusarium moniliforme on seed germination rate and germination potential decreased most significantly(p<0.05). Seedlings on the seedling growth of most notably were Fusarium oxysporum, Fusarium moniliforme, Fusarium solani. Seedling root growth on the most obvious is Fusarium oxysporum, Fusarium solani. Fusarium moniliforme on the pathogenicity of seedlings and roots are the largest. Second, the pathogenicity of the vaccine is more obvious sainfoin and Ascochyta onobrychidis and the pathogenicity of the root is obvious Verticillium sp.