Effect Of Condensed Tannin On Ruminal Fermentation And Nutrient Digestion

In recent years, the study found that the rumen utilization performance for protein was small. These are duo to the degradation rate of the plant protein is faster than the microprotein synthesis rate which is produced with ammine, resulting in the rumen microbes take advantage of the plant proteins. Therefore, how to raise the utilization rate of the protein became the hot issue of ruminant animal studies. Condensed tannins is a multi-phenolic compounds, can be combined with proteins to make the protein less susceptible to degradation, protect proteins. In this study, according to this feature, the effects of different concentrations of condensed tannins on the dietary protein protection in vitro or in vivo were determined. These provided a reference for improving the utilization of ruminant animal feed proteinEffect of condensed tannin on dietary protein, ruminal fermentation and rumen bacteria in vitroThe objective of this experiment was to study effects of condensed tannin on rumen fermentation and rumen degradability of protein in vitro. The experiment was provided six concentrations of condensed tannin(0%,1%,2%,3%,4%,5%/DM). Investigated the influence of the contents of the PCT, FCT, NH3-N, MCP, the pH value and the gas production. To sieve the highest concentration of condensed tannin.The results showed that:the content of PCT in group 3%, group 4%, group 5% were higher compared to the group 1%, group 2%(p<0.05).The little effect between the condensed tannin and the cellulose, and the content of FCT had no differences between the experimental groups (p>0.05). The gas production in group 3%, group 4%, group 5% were lower compared to the group 1%, group 2%(p<0.05), The value of pH didn't vary markedly in the experimental groups (p>0.05).The content of NH3-N in group 0%, group 1%, group 2%, group 3% were higher than group 4% and 5%(p<0.05). The content of MCP in group 0%, group 1%, group 2%, group 3% were higher than group 4% and 5%(p<0.05).The conclusion was that the content of condensed tannin shouldn't high to 3%.Effect of condensed tannin on ruminal fermentation, rumen bacteria and nutrient digestionWith the conclusion of the first experiment, the project was 3×3 completely design, there were three treatments:control group, group 1.5% and group 3%, condensed tannin were added to the concentrate at 1.5% and 3% levels of the diet (concentrate basis), to detect the effects of pH value, total nitrogen, NH3-N, urea nitrogen, protein nitrogen, VFA and digestibility.The results showed that:The value of pH didn't vary markedly in the experimental groups (p>0.05).So was the content of the total nitrogen, but the treatments stable. The content of the NH3-N in control group was higher than the treatments, there were vary markedly at 3h. The content of urea nitrogen had no differences between the experimental groups (p>0.05) after diet, but the treatment groups changed weakly. The content of protein nitrogen in control group was higher than group 3%(p<0.05). there were no differences at other times (p>0.05). The colony counts of proteolytic bacteria in treatment groups were lower than control group (p<0.05). The colony counts of cellulolytic bacteria, total viable bacteria and amylolytic bacteria were lower than control group,but no differences (p>0.05). The content of VFA in treatment groups were lower than control group, but no differences (p>0.05). The degradability of DM, OM, EE, NDF, ADF had no differences between the experimental groups (p>0.05). The degradability of CP in group 3% was higher than control group (p<0.05). The conclusion was that:the additive level of condensed tannin at 3% had no differences to ruminal fermentation and ruminal microbial, but increased the degradability of CP vary markedly.