Study On Determination Of 100 Pesticide Residues In Fructus Luch By Gas Chromatogramy-Mass Spectrometry And Three Aromatic Traditional Chinese Medicine Quality Research

As the dried fruit of nightshade Lucium barbarum L., Fructus Lycii is a Traditional Chinese Medicine that can be commonly-used for food or for nourishing. This study has developed a method for the determination of pesticide residues in the Fructus Lycii. The study is extraordinarily essential to ensure the medicine safety and control quality of Fructus Lycii.A new method for determine the pesticides in Fructus Lycii was developed. The detection was using the technology of GC-MS-SIM. and GC-MS-MS. The sample were extracted with acetonitrile using ultrasonic extraction, then cleaned up by a column packed with Carb/NH2.The capillary column was VF-5MS and samples were separated by the temperature program. The quantities of pesticide residues were calculated by external standard method. For the GC-MS-SIM method, pesticides were positively confirmed by retention time and ion ratios. For GC-MS-MS method, pesticides were positively confirmed by the precursor ion and product ions. Use the method, The average recoveries ranged from 75.10-121.77%and RSD≤15.0%, at three spiked mixed pesticide levels, which accorded with the requirement of pesticide residues determination. The method is easy in operation, accurate in determining and has good sensitivity and repeatability. The method can be applied to more species fruit TCMs.In addition, the quality control of the volatile oil medical plants such as Herba Eupatorii, Herba Schizonepetae and Herba Pogostemonis have also been studied. The main constitutes of three TCMs have been recognized, and the analysis methods of Eupatorium fortunei Turcz. and Schizonepeta tenuisfolia Briq. have also been set up. The fingerprints of Schizonepeta tenuisfolia Briq. and Pogostemon cablin (Blanco) Benth. have been studied, mutual mode of chromatographic fingerprints of the two TCMs have been established, seperately.