| Lycii Fructus is the dried fruit of Lycium barbarum L. It has the function of nourishing liver and kidney, benefiting shrewd eyes. It is the first two medicines approved by the Ministry of Health to be used as both medicine and food. The Lycii Fructus that is big, red, seedless, soft and sweet is considered the best, among which the color and the appearance are the most important standards. Color, as a quality evaluation index, is one of Chinese traditional medicine quality evaluation characteristics, the color of medicinal materials related to its internal material base, each medicinal materials often have their particular colors, traditional quality evaluation, such as Rhizoma Coptidis Color Yellow, salvia miltiorrhiza Color Red, Radix Arnebiae seu lithospermi (To color, violet wait for medicinal materials quality. Quantity is optimal. Herbs appearance color is given priority to with traditional experience to identify, strong subjectivity, describe fuzzy. In recent years, colorimetry was gradually introduced into traditional Chinese medicine quality evaluation field, for the CIE chromaticity commonly used evaluation system. The 1976 uniform color. Empty, namely CIE-Lab system. System, for yan To express the color. Now the evaluation system has been widely used in traditional Chinese medicine (TCM) in the field of color measurement, can objective quantitative medicinal materials, color for easy "color changing" Chinese herbal medicine storage maintenance is of great significance.Because Lycii Fructus is rich in sugar, it is easy for the color to change in the storage process. It is one of the representatives of the Chinese medicinal materials which are easy to change their colors. Color change is the most important evaluation indicators of quality deterioration for medlar. However, the correlation between the color change and its inherent material base of Lycii Fructus have not been reported in literature, the early stage of the team has to packaging materials, storage conditions, effect of Lycii Fructus quality. Research. Taking medlar as the research object, the stable constant temperature box, under the condition of high temperature, high humidity, every 10 days sampling, accelerated experiment 2 consecutive month, the first objective quantitative medlar medicine color appearance, inner material foundation changes with correlation analysis, to explore Lycii Fructus storage "color changing" phenomenon in the process of scientific connotation. It is of high significance to research medlar storage for maintenance of product as well as the quality evaluation. Here are the main research contents and results.1. The preliminary research of objective and quantitative color change of Lycii FructusTraditional identification mainly depends on human senses. Although this method is intuitive and convenient, it also has strong subjectivity and fuzziness. This method requires serious training and rich experience. Which it hard to bring its inheritance and application difficulties. This paper USES light color measurement instrument for the first time the appearance of color in the process of 3 batches of medlar storage for scientific objective quantitative, with L*, a*, b* value of color expression, which L* is brightness value, the black at the bottom, white at the top; a* value for red green degree,+-a* said red,-a* green; b* degrees for the yellow and blue value,+b* said yellow,-b* said blue.Results show that the impression officer to assess three batch of medlar appearance by the bright red color in the process of gradually become dark brown, light color measurement instrument to determine the L *, a*. b* value overall showed a trend of decrease, L* values decrease rate were 27.8%.34.9%.27.5% in turn. a* value reduced rate of 63.7%.78.3%.58.3%. b* value decline rate were 58.6%,75.6%,52.3% in turn.It showed that Chinese medicinal materials in the storage process color brightness, partial red, yellow are less.The results of the research provides evidences for the identification of traditional Chinese medicine, judging the color change from the traditional experience to objective quantitative evaluation.2. The research of Internal material basis change of Lycii Fructus in "color changing"2.1 The research on sugars change of Lycii Fructus in "color changing"Lycii Fructus is rich in sugars, and as we know, sugars, in certain conditions, such as temperature, humidity and so on, can be combined with Amino acids which Maillard reaction, as a result of which, its appearance color changes from red to dark brown. At the same time, sugars itself can also occur degradation reaction.5-HMF is considered to be the product of the two reactions. This article first systematically study medlar storage sugars in the process of change, and it was established medlar medicinal material of two kinds of water-soluble monosaccharides (fructose and glucose) and determined the content of 5-HMF results by HPLC. Experimental results show that, with medlar color from the bright red in the process of gradually become dark brown, LBP and glucose and fructose are overall all showed a trend of decrease, the LBP content is lower, reduce the rate of 43% to 57%, fructose content lower, reduce the rate of 25% to31%, glucose content is lower, reduce the rate of 32% to 55%. The content of 5-HMF results showed a trend of obvious rise, change color after medlar 5-HMF results in the increase of content of 51-114 times before color. Show that Lycii Fructus sugars occur in the process of storage wheat or degradation reaction, rudd generate 5-HMF results. Results can be preliminarily determined 5-HMF associated with c appearance color changes in the process of storage, to clarify medlar "color changing" material base to provide the scientific basis.2.2 The research of Pigment ingredients change of Lycii Fructus in "color changing"Lycii Fructus pigments class composition is mainly divided into two categories, fat-soluble carotenoid compounds and water-soluble polyphenols, flavonoids compound. The early stage of The team of carotenoids in Lycii Fructus was established, total phenolic acid content determination method, based on this, this article use ultraviolet-visible spectrophotometer (UV) determination of carotenoids and flavonoids in medlar storage process and total phenolic acid content changes, The results show that, as Lycii Fructus color from red to dark brown in The process, including fat-soluble pigments carotenoid content showed a trend of decrease, falling at a rate of 76%to 87%; Water-soluble pigment flavonoids content is basic stable, no obvious changes, and total phenolic acid content increased, medlar total phenolic acid content increased after color change to discoloration before The content of 2.2 to 2.4 times. It is basically acknowledged that carotenoids, total phenolic acids are the material base of the color change of Lycii Fructus in the process of storage.3. The correlation between color and compositions change of Lycii Fructus in "color changing"The color of medicine is considered one of the quality evaluation indexes. This paper, based on the colorimetric analysis method for the first time, makes the medlar colof quantitative. Using SPSS analysis software, and medicinal ingredients like sugars, pigment content in the correlation analysis. preliminary show medlar in appearance and its internal ingredients like sugars, pigment content has a close correlation. Of medlar appearance color L* a* b* values and LBP, monosaccharide content show strong positive correlation (P< 0.05). and negatively correlated to the content of 5-HMF results (P< 0.05). suggesting that the Lycii Fructus sugars occur in the process of storage wheat reaction or degradation reaction; Medlar appearance color value and carotenoid content were significantly positive correlation (P< 0.01), and total phenolic acid content was significantly negative correlation (P< 0.01), and there was no significant correlation with the content of total flavonoids.lt shows that carotenoids, total phenolic acids are Lycii Fructus color material foundation, which clarifies Lycii Fructus"color changing" scientific connotation is of great significance. |
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