The Organs Structure Feature And The Accumulation Laws Of The Main Medcinal Component Study On The Prunella Vulgaris Lnn.

Prunella vulgaris Inn is perennial herb of the Labiatae, and make dry ears as medcine, owning Liver eyesight and swelling Sanjie effect.The egasporogenesis,microsporogenesis and development of male and female gametophytes of Prunella vulgaris Linn were studied using method of paraffin section.this experiment studied:the structural characteristics.of the Prunella vulgaris. vegetative organs and reproductive organs. The accumulation laws of the total flavones,ursolic acid,oleanolic acid,RosA,and caffeic acid with the spectrophotometry, and compared the medcinal gredients of the common medcinal part ears and also the different medcinal composition content between Ning shan and yangling.The result provided theory basis for the identification of Microstructure, biology reproductive feature, rational development and utilization, to determine the best harvest time and search for the new drug source area.The main conclution were as follows:1. There were three layers from the outside to inside in turn of the Prunella vulgaris Inn primary root:epidermis, cortex, vascular cambium.Threr were pericycle,primary xylem,primary phloem in the vascular cambium of the primary root structure, The primary xylem had four beams, the four prototype roots, with normal growth and secondary structure. The stem cross-section leaving it square, they are cortex and vascular cylinder, vascular cylinder in vascular bundles from outside to inside, pulp and pith ray in the prunella stem primary structure from the outer epidermis in turn。The prunella stem secondary growth and secondary structure:the secondary xylem cells, the secondary phloem cells, there are catheter, tracheid and some wood parenchyma in the xylem. the prunella leaf internal structure contained epidermis mesophyll and veins these three basic components, the Palisade tissue and spongy tissue was clear。The pores were ususlly distributed in the lower leaves.2. The Prunella vulgaris ovule was anatropous, lacoste was thin nucellus, Polygonum type embryo sac, The anther had four pollen sacs, The anther wall consists of epidermis, endothecium, middle and tapetal component, each section has a layer of cells. The tapetum type was glandular tapetum. The microspore mother cell meiosis owned to simultaneous,the tetrad was tetrahedral, the mature pollen grains are 2-cellthe. The big spore mother cell, Tetrads linear or "T" type.3. The determination of the total flavonoids in Prunella vulgaris:The accumulation of flavonoids in the Prunella vegetative organs root, stem and leaf was more and more with the flowers development and the fruits formation。The flavonoids content during buds stage were all low, the flavonoid content got to the highest in the fruit maturity and fruit in the dry period.4. The determination of ursolic acid in Prunella vulgaris:the Ursolic acid content in the root was gradually increased from buds until fruit maturity stage,the content during fruit dry stage was lower, there were two peak periods the Ursolic acid content in the stem, the buds and the fruit maturity stage, and the former higher than the latter.the Ursolic acid content got to the highest when the fruit maturity stage in the leaves and flowers.5. The dermination of the oleanolic acid content in Prunella vulgaris:the oleanolic acid content in Prunella vulgaris roots were higher than the other three parts (stems, leaves, flowers).The oleanolic acid content of the root in the early flowering stage was highest, after the early flowering stage, oleanolic acid content began declining gradually.The oleanolic acid content in stems at flowering and early flowering stage are all high,and the early flowering stage higher than the final flowering.Theoleanolic acid content of leaves got to the highest at the end of flowering, while the oleanolic acid content of the flowers got to the highest in the flowering stage, decreased gradually after flowering.6. The determination of rosmarinicacid content with spectrophotometric method:the Rosmarinic acid content in flowers was higher than the other three parts (roots, stems, leaves), and the highest content times of rosmarinic acid in different parts varied.The rosmarinic acid content in the Roots got to the highest at the end of flowering stage,the rosmarinic acid content in the stems get to the highest at the fruit maturity stage and the rosmarinic acid content in leaves get to the highest during buds stage, the content of rosmarinic acid in the early flowers and fruit maturity stage got to the highest.7. The determination of caffeic acid content:the caffeic acid content in flowers was the highest, followed by leaf, stem and root. The caffeic acid content in roots at different growth stages changed a little, the caffeic acid content in stems,leaves and flowers stage got to the highest at the fruit maturity.