Key Technologies Of Protecting He'nan Long-tail Sheep

He'nan Long-tail Sheep is a eminent local variety dilution.The experiment observed the spermatozoon, superovulation,fibroblasts cultured in vitro,to achieve presercation of He'nan Long-tail Sheep's variety and provide technical support for gene bank establishing.1 Test of straw frozen semen of He'nan long-tail sheep Collect the semen of He'nan long-tail sheep, comparing the effect of different frozen diluent ingredient, different glycerin concentration, different levels of the egg yolk. The result indicates that the activity of 8.25 g lactose and 2.5 g glucose combined was 0.38±0.24,deformity rates was 13.43%±0.36. The optimum concentration of glycerin and egg yolk were 6% and 20%.2 Test of superovulation of He'nan Long-tail Han Sheep To study the effects of hormone, season, insemination method and repeated superovulation on superovulatory response in He'nan Long-tail Han Sheep, Long-tail han sheep were superovulated. The results demonstrated that the average CL(15.76±4.53) and average embryos (12.76±5.18)of Canada's FSH were significantly more than that Newzealand's FSH(11.78±3.62, 8.78±2.67), the average CL(18.11±4.00), average embryos(14.84±3.30) and average available embryos(5.21±1.72)of winter were significantly more than that spring(13.44±2.95, 9.44±2.09, 3.78±1.93) and autumn(11.61±3.05, 9.56±2.57, 2.72±2.08), the average CL (18.27±9.46)and average embryos(14.53±11.00) of 350mg dose of Canada'FSH were significantly more than that 300mg(12.23±4.81, 10.23±4.90), The average available embryos (4.68±2.61)of intrauterine cervices insemination were significantly more than aginal insemination (1.89±4.14),There was no significant difference among the first superovulation, the second superovulation and the third superovulation. After freezing, embryos form a complete sampling rate of 100% growth rate of 75%.3 Isolation and culture of He'nan Long-tail Han Sheep fibroblast The primary cultured and subculture of He'nan long-tail han sheep fibroblast were cultured by tissue patch. The result indicates that it was successful to culture He'nan long-tail han sheep fibroblast by tissue patch. The primary culturedskin cells were fibroblast epithelial cells, fibroblasts can be purified by subculture. The day of skin fibroblasts of He'nan long-tail han sheep to grow incubation period, logarithmic growth phase and plateau phase were 0~5d,5~8d,8~10d.when the fibroblasts were cultured for 5 passage,the chromosme karyotype was normal...