| Frozen semen technology uses liquid nitrogen, dry ice as a frozen source, and saves at extremely low temperatures right after a special processing of semen so that it can achieve a long-term preservation purpose.Apparently, natural fecundity of sheep has been low, and a wise way to accelerate the pace of species of breeding and improvement is to maximize the utilization of superior male livestock, and enlarge the scale of the development of sheep production. Therefore, the way of frozen semen among breeding stock to preserve some genetic information to promote sheep cooling technology is very important.Based on this situation above, this study is a further trail on frozen sheep semen dilution for further exploration to improve post-thaw sperm viability, acrosomal integrity, and conception rate et cetera. In this way we can ensure the fresh utilization and avoid waste of resources. Furthermore, we can stabilize the successful formula of its vitality. The main results of this study and conclusions are as follows:1. Different diluents on the cryopreservation of sperm of different, with ethylene glycol as the cryoprotectant after thawing activity was high, the effect significantly, reaching 0.43±0.028.2.Different thawing temperature on sperm motility were significantly affected, at 42℃thaw viability of sperm after thawing the highest, reaching 0.40±0.02.3.Selected by experiment the best added diluents on sperm EDTA and Tris good protection.4.Twice the diluted sperm after thawing the most active, effective sperm count more conducive to sperm survival.5.This study sheep cooling technology to further promote and reference for other researchers to provide a better platform.
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