Isolation And Screening Of Heterofermentative Lactic Bacteria From Gramineas And The Effect Of Adding It On Silage Fermentation Quality And Aerobic Stability

The object is that selected epiphytic Heterofermentative Lactic Bacteria from gramineous grass Bromus inermis Leyss, Isolated, identified and selected excellent Heterofermentative Lactic Bacteria strains. Then the strain or strains were applied as inoculants, investigated the effect on fermentation characteristics and aerobic stability. It is a valuable study how to improve graminouse grass feed value and feeding effect.The results showed that general bacteria, yeast, mold are main micro epiphytic on Bromus inermis Leyss, and their levels were more than 106cfu/gFM (colonies per gram of fresh grass with units), compared with the number of lactic acid bacteria the highest content is less than 5×104cfu/gFM. So in order to obtain high-quality silage,we must add a certain degree of lactic acid bacteria to dominate the fermentation.Eight Heterofermentative Lactic Bacteria strains were isolated and selected 2 excellent strains from it via morphologic, physiology, biochemistry and carbohydrate fermentation, They were Lactobacillus buchneri, they possessed some good quality, such as, rapidly fermenting, extensive growth in different temperature, facilely utilized carbohydrate to formlactic acid, Heterofermentative Lactic Bacteria. Their multiplication was rapid, the highest alive colony forming unit was about 109 cfu/ml broth.A study was conducted to investigate the effect of applying Lactobacillus Plantarum,Enterococcus Mundtti and Lactobacillus buchneri on fermentation characteristics and aerobic stability of Bromus inermis Leyss. There were four treatments as followed: the control (no additives) (Con), the L.buchneri (he), the L.Plantarum and E.Mundtii(ho) and the, L.buchneri,L.Plantarum and E.Mundti (he+ho).In anaerobic fermentation stage, L.Plantarum and E.Mundtii can contribute to improved the content of lactic acid, reduced acetic acid, ammonia nitrogen, L.buchneri reduced the content of lactic acid, ammonia nitrogen, but increased acetic acid, L.buchneri,L.Plantarum and E.Mundti improved the content of lactic acid, acetic acid, decreased ammonia nitrogen content. Add different types of lactic acid bacteria can inhibit mold and butyric, reduced CP, WSC loss, but not affected the NDF, ADF, Ash contents.In the aerobic exposure period, L.Plantarum and E.Mundtii treatment group pH increased rapidly, lactic acid, acetic acid, WSC content decreased rapidly. The number of yeasts and molds increased, aerobic stability had no significant difference (p>0.05) between L.Plantarum and E.Mundtii treatment and the control. Through out the trial period L.buchneri treatment group pH changed very slow, The loss of lactic acid, acetic acid, WSC content was significantly lower than other three treatment groups (p<0.05), the number of yeasts and molds proliferation very slow during exposure to air. L.buchneri,L.Plantarum and E.Mundti treatment group lactic acid, acetic acid was significantly higher than the control, the time of pH began to rise and the time of WSC decrease was late than the control , the number of yeasts and molds proliferation very slow during exposure to air.