| In this experiment, Hu sheep was the researched object and Poll Dorset,Texel,Suffolk and Xiao Wei Han sheep were the referenced groups.Methods of candidate gene,PCR-RFLP, PCR-SSCP and DNA sequencing techniques were applied to analyze the genetic variation of partly GH and MSTN gene fragments and approach the relation between genetic variation and partly growth traits and analyse the possibiliti of GH and MSTN gene to be the candidate genes that effecting the growth and development of muscle in Hu sheep.At the same time, investigate the allocation and express abundance of the two genes at different stages of days in muscle in Hu sheep.The purpose is to reveal the genetic technology of growth in muscle in Hu sheep by using candidate gene method and provide genetic information for the selection of muscle growth in Hu sheep. The results were as follows:1 Association analysis of genetic polymorphism and early muscle growth traits of GH and MSTN gene in Hu sheep(1) Association analysis of genetic polymorphism and early muscle growth traits of GH gene in Hu sheepA single nucleotide substitution of C to T was found on the GH Extron-1 site. There was a single nucleotide substitution of C to G was found on the GH Extron-2 site and a A to G single nucleotide substitution was found on the GH Extron-3 site. There was also a single nucleotide substitution of A to G was found on the GH Extron -4 site. The result of chisquare-suitability test showed:site of GH Extron-3 was in extreme imbalance, site of GH Extron-1 was significant balanced condition and other sites were balanced condition.There were significant differences on Birth weight and 6-month-age weight between the two genotypes Aa and AB of GH Extron-1. There was significant difference on Post weaning weight for CC genotype of GH Extron-2 than other three grnotypes. There was no significant difference on the three growth traits for GH Extron-3 between GG and GH genetypes and GH Extron-4 between MM and LM genetypes.GH genetype in Extron-3 and LM genetype in Extron-4 were advantageous genetype for growth traits.(2) Association analysis of genetic polymorphism and early muscle growth traits of MSTN gene in Hu sheepA single nucleotide substitution of A to G was found in MSTN promoter site,a A to G single nucleotide substitution was found in Extron-2 site,a single nucleotide substitution of G to T was found in 3′control region of MSTN site. The result of chisquare-suitability test showed: 3′control region site was in extreme imbalance and other sites were in balanced condition. There were significant differences on Post weaning weight and 6-month-age weight between the three genotypes OO,OP and PP of MSTN promoter.There were significant differences on Post weaning weight and 6-month-age weight between the two genotypes XX and XW site of MSTN Extron-2.There was no significant difference on the three growth traits between the two genotypes YY and YZ of 3′control region site of MSTN. YY genetype in 3′control region and PP genetype in promoter were advantageous genetype for growth traits.2 Association analysis between GH,MSTN gene expression and butcher traits in Hu sheep (1)Expression of GH gene at different early days in Hu sheepThere was fundamental same tendency of developed changes of GH gene between rams and ewes.Followed by the increase of days after birth,the relative expression levels took on descend tendency in muscular tissue.On the 2 days,there was the highest expression level.For rams:there were significant difference between 60,180 days and other days.Among that,there were extreme significant difference between 60,90,180 days and other days.For ewes:there were significant difference between 30,60days and 120,180 days and other days.Among that, there were extreme significant difference between 30,60,90,180 days and other days. Between rams and ewes,there was significant difference on 180 days and extreme significant difference between 2,30,60,90 and 120 days.(2)Expression of MSTN gene at different early days in Hu sheepFollowed by the increase of days after birth,the relative expression levels of MSTN gene in muscular tissue took on the tendency of first increased and then descended. There was the highest expression level on the 60 days. For rams: there were significant difference between defferent days.Among that, there were extreme significant difference between 30,120 days and other days.For ewes:there were both significant and extreme significant difference between 30,90 days and 60,180 days and other days. Between rams and ewes,there was significant difference between 60 and 180 days and extreme significant difference between 30,90 and 120 days.(3) Correlation analysis of expression abundance of GH and MSTN gene and butcher traitThe results of correlation analysis showed that: there were extreme significant negative correlation between GH gene expression and MSTN gene expression.There were extreme significant negative correlation between GH gene expression and Live weight,Carcass weight and Meat percentage.At the same time, there was significant correlation between MSTN gene expression and Meat percentage.There was no significant correlation between MSTN gene expression and Live weight and Carcass weight.
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